FGFR2 Kit ELISA

N° du produit ABIN4886102

Détail du produit FGFR2 Kit ELISA

Antigène: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Épitope: pTyr

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Phosphotyrosine FGFR2 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FGFR2 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-FGFR2.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))

Autre désignation: FGFR2 (FGFR2 Produits)

Synonymes: FGFR2 Kit ELISA, FGFR2IIIb Kit ELISA, bek Kit ELISA, bfr-1 Kit ELISA, cd332 Kit ELISA, cek3 Kit ELISA, cfd1 Kit ELISA, ect1 Kit ELISA, fgfr-2 Kit ELISA, jws Kit ELISA, k-sam Kit ELISA, kgfr Kit ELISA, tk14 Kit ELISA, tk25 Kit ELISA, xfgfr2 Kit ELISA, DKFZp469H1521 Kit ELISA, BBDS Kit ELISA, BEK Kit ELISA, BFR-1 Kit ELISA, CD332 Kit ELISA, CEK3 Kit ELISA, CFD1 Kit ELISA, ECT1 Kit ELISA, JWS Kit ELISA, K-SAM Kit ELISA, KGFR Kit ELISA, TK14 Kit ELISA, TK25 Kit ELISA, AU043015 Kit ELISA, AW556123 Kit ELISA, Bek Kit ELISA, Fgfr-2 Kit ELISA, Fgfr-7 Kit ELISA, Fgfr7 Kit ELISA, KGFRTr Kit ELISA, svs Kit ELISA, FGF-R Kit ELISA, fgfr72IIIb Kit ELISA, FGFR Kit ELISA, fc56c05 Kit ELISA, wu:fc56c05 Kit ELISA, fibroblast growth factor receptor 2 Kit ELISA, fibroblast growth factor receptor 2 L homeolog Kit ELISA, FGFR2 Kit ELISA, LOC100084641 Kit ELISA, fgfr2 Kit ELISA, Fgfr2 Kit ELISA, fgfr2.L Kit ELISA

ID gène: 2263

UniProt: P21802

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding