FGFR2 Kit ELISA
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- Antigène Voir toutes FGFR2 Kits ELISA
- FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
- Épitope
- pTyr
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Human Phosphotyrosine FGFR2 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FGFR2 in lysate samples.
- Type d'échantillon
- Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-FGFR2.
- Attributs du produit
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- Rapidly measure phosphorylated protein in lysates
- Screen numerous different cell lysates without performing a Western Blot analysis
- Minimal hands-on time, convenient, and non-radioactive material
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Biotinylated Anti-Phosphotyrosine Antibody
- Stop Solution
- Assay Diluent(s)
- Positive Control Sample
- Lysis Buffer
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
- Date de péremption
- 6 months
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- Antigène Voir toutes FGFR2 Kits ELISA
- FGFR2 (Fibroblast Growth Factor Receptor 2 (FGFR2))
- Autre désignation
- FGFR2 (FGFR2 Produits)
- Synonymes
- FGFR2 Kit ELISA, FGFR2IIIb Kit ELISA, bek Kit ELISA, bfr-1 Kit ELISA, cd332 Kit ELISA, cek3 Kit ELISA, cfd1 Kit ELISA, ect1 Kit ELISA, fgfr-2 Kit ELISA, jws Kit ELISA, k-sam Kit ELISA, kgfr Kit ELISA, tk14 Kit ELISA, tk25 Kit ELISA, xfgfr2 Kit ELISA, DKFZp469H1521 Kit ELISA, BBDS Kit ELISA, BEK Kit ELISA, BFR-1 Kit ELISA, CD332 Kit ELISA, CEK3 Kit ELISA, CFD1 Kit ELISA, ECT1 Kit ELISA, JWS Kit ELISA, K-SAM Kit ELISA, KGFR Kit ELISA, TK14 Kit ELISA, TK25 Kit ELISA, AU043015 Kit ELISA, AW556123 Kit ELISA, Bek Kit ELISA, Fgfr-2 Kit ELISA, Fgfr-7 Kit ELISA, Fgfr7 Kit ELISA, KGFRTr Kit ELISA, svs Kit ELISA, FGF-R Kit ELISA, fgfr72IIIb Kit ELISA, FGFR Kit ELISA, fc56c05 Kit ELISA, wu:fc56c05 Kit ELISA, fibroblast growth factor receptor 2 Kit ELISA, fibroblast growth factor receptor 2 L homeolog Kit ELISA, FGFR2 Kit ELISA, LOC100084641 Kit ELISA, fgfr2 Kit ELISA, Fgfr2 Kit ELISA, fgfr2.L Kit ELISA
- ID gène
- 2263
- UniProt
- P21802
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding
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