HDAC4 Kit ELISA

N° du produit ABIN4886126

Détail du produit HDAC4 Kit ELISA

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Épitope: pSer632, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-HDAC4 (S632) and Total HDAC4 ELISA Kit. This assay semi-quantitatively measures phophorylated HDAC4 (Ser632) and Total HDAC4 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human HDAC4 phosphorylated at site Serine-632 and total HDAC4.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Autre désignation: HDAC4 (HDAC4 Produits)

Synonymes: CG1770 Kit ELISA, DHDAC4 Kit ELISA, Dmel\\CG1770 Kit ELISA, GC1770 Kit ELISA, HDAC Kit ELISA, HDAC4a Kit ELISA, dHDAC4 Kit ELISA, dmHDA405 Kit ELISA, hdac4 Kit ELISA, dana_GLEANR_19966 Kit ELISA, DanaGF18710 Kit ELISA, GF18710 Kit ELISA, HDAC4 Kit ELISA, AHO3 Kit ELISA, BDMR Kit ELISA, HA6116 Kit ELISA, HD4 Kit ELISA, HDAC-4 Kit ELISA, HDAC-A Kit ELISA, HDACA Kit ELISA, 4932408F19Rik Kit ELISA, AI047285 Kit ELISA, wu:fa96d08 Kit ELISA, wu:fc56f08 Kit ELISA, zgc:152701 Kit ELISA, histone deacetylase 4 Kit ELISA, hypothetical protein Kit ELISA, Histone deacetylase 4 Kit ELISA, GF18710 gene product from transcript GF18710-RC Kit ELISA, PTRG_03699 Kit ELISA, PGTG_15512 Kit ELISA, HDAC4 Kit ELISA, Dana\GF18710 Kit ELISA, Hdac4 Kit ELISA, hdac4 Kit ELISA

ID gène: 25698

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process