Insulin Receptor Kit ELISA

N° du produit ABIN4886144

Détail du produit Insulin Receptor Kit ELISA

Antigène: Insulin Receptor (INSR)

Épitope: pTyr1189, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-INSR (Y1189) and Total INSR ELISA Kit. This assay semi-quantitatively measures phophorylated INSR (Tyr1189) and Total INSR in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human INSR phosphorylated at site Tyrosine-1189 and total INSR.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: Insulin Receptor (INSR)

Autre désignation: Insulin R (INSR Produits)

Synonymes: CD220 Kit ELISA, HHF5 Kit ELISA, 4932439J01Rik Kit ELISA, D630014A15Rik Kit ELISA, IR Kit ELISA, IR-A Kit ELISA, IR-B Kit ELISA, 18402 Kit ELISA, CG18402 Kit ELISA, DIHR Kit ELISA, DILR Kit ELISA, DIR Kit ELISA, DIRH Kit ELISA, DIRbeta Kit ELISA, DInR Kit ELISA, DInr Kit ELISA, Dir-a Kit ELISA, Dir-b Kit ELISA, Dmel\\CG18402 Kit ELISA, INR Kit ELISA, INS Kit ELISA, Inr Kit ELISA, Inr-alpha Kit ELISA, Inr-beta Kit ELISA, InsR Kit ELISA, dINR Kit ELISA, dIR Kit ELISA, dIRH Kit ELISA, dInR Kit ELISA, dInr Kit ELISA, dInsR Kit ELISA, dinr Kit ELISA, dir Kit ELISA, er10 Kit ELISA, inr Kit ELISA, insulin/insulin-like growth factor receptor Kit ELISA, l(3)05545 Kit ELISA, l(3)93Dj Kit ELISA, l(3)er10 Kit ELISA, lnR Kit ELISA, ir-A Kit ELISA, CTK-1 Kit ELISA, ir Kit ELISA, INSR Kit ELISA, NV14476 Kit ELISA, cd220 Kit ELISA, hhf5 Kit ELISA, insulin receptor Kit ELISA, Insulin-like receptor Kit ELISA, insulin receptor L homeolog Kit ELISA, INSR Kit ELISA, Insr Kit ELISA, InR Kit ELISA, LOC100122567 Kit ELISA, LOC100451802 Kit ELISA, insr.L Kit ELISA

ID gène: 3643

UniProt: P06213

Pathways: Signalisation NF-kappaB, Signalisation RTK, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity