Midkine Kit ELISA

N° du produit ABIN4886204

Détail du produit Midkine Kit ELISA

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Épitope: pTyr

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Phosphotyrosine MEK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine MEK in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-MEK.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Autre désignation: MEK (MDK Produits)

Synonymes: ARAP Kit ELISA, MK Kit ELISA, NEGF2 Kit ELISA, Mek Kit ELISA, MK-B Kit ELISA, PTF-alpha-1 Kit ELISA, mdk Kit ELISA, midkine Kit ELISA, negf2 Kit ELISA, RIHB Kit ELISA, cb518 Kit ELISA, fb79b05 Kit ELISA, fj33b02 Kit ELISA, fj48a12 Kit ELISA, mdk2 Kit ELISA, plei2 Kit ELISA, wu:fb79b05 Kit ELISA, wu:fj33b02 Kit ELISA, wu:fj48a12 Kit ELISA, MK-A Kit ELISA, PTF-alpha-2 Kit ELISA, X-PTF-alpha1 Kit ELISA, XMK Kit ELISA, mdk-a Kit ELISA, mdk-b Kit ELISA, midkine Kit ELISA, midkine L homeolog Kit ELISA, midkine (neurite growth-promoting factor 2) Kit ELISA, midkine b Kit ELISA, midkine S homeolog Kit ELISA, MDK Kit ELISA, Mdk Kit ELISA, mdk.L Kit ELISA, mdkb Kit ELISA, mdk.S Kit ELISA

ID gène: 5604

UniProt: Q02750

Pathways: Signalisation RTK, M Phase, Skeletal Muscle Fiber Development