NGFR Kit ELISA

N° du produit ABIN4886225

Détail du produit NGFR Kit ELISA

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Épitope: pTyr

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human Phosphotyrosine NGFR ELISA Kit. This assay semi-quantitatively measures phosphotyrosine NGFR in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-NGFR.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: NGFR (Nerve Growth Factor Receptor (NGFR))

Autre désignation: NGFR (NGFR Produits)

Synonymes: ngfr Kit ELISA, wu:fb38c02 Kit ELISA, wu:fc10h01 Kit ELISA, CD271 Kit ELISA, Gp80-LNGFR Kit ELISA, TNFRSF16 Kit ELISA, p75(NTR) Kit ELISA, p75NTR Kit ELISA, LNGFR Kit ELISA, RNNGFRR Kit ELISA, Tnfrsf16 Kit ELISA, p75 Kit ELISA, NRH1b Kit ELISA, fullback Kit ELISA, nrh1 Kit ELISA, p75ntr Kit ELISA, tnfrsf16 Kit ELISA, p75NGFR Kit ELISA, nerve growth factor receptor Kit ELISA, neurotrophin receptor associated death domain Kit ELISA, nerve growth factor receptor S homeolog Kit ELISA, nerve growth factor receptor (TNFR superfamily, member 16) Kit ELISA, NGFR Kit ELISA, nradd Kit ELISA, Ngfr Kit ELISA, ngfr.S Kit ELISA

ID gène: 5715

UniProt: O00233

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding