Ret Proto-Oncogene Kit ELISA

N° du produit ABIN4886261

Détail du produit Ret Proto-Oncogene Kit ELISA

Antigène: Ret Proto-Oncogene (RET)

Épitope: pTyr

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phosphotyrosine RET ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated RET in cell lysate samples.

Type d'échantillon: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes human RET phosphotyrosine.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: Ret Proto-Oncogene (RET)

Autre désignation: RET (RET Produits)

Synonymes: c-ret Kit ELISA, cret Kit ELISA, etID315074.13 Kit ELISA, ret1 Kit ELISA, wu:fd13h01 Kit ELISA, X-ret Kit ELISA, ret-A Kit ELISA, xret Kit ELISA, RET Kit ELISA, MTC1 Kit ELISA, CDHF12 Kit ELISA, CDHR16 Kit ELISA, HSCR1 Kit ELISA, MEN2A Kit ELISA, MEN2B Kit ELISA, PTC Kit ELISA, RET-ELE1 Kit ELISA, RET51 Kit ELISA, RET9 Kit ELISA, c-Ret Kit ELISA, ret proto-oncogene receptor tyrosine kinase Kit ELISA, ret proto-oncogene S homeolog Kit ELISA, ret proto-oncogene Kit ELISA, ret Kit ELISA, ret.S Kit ELISA, RET Kit ELISA, Ret Kit ELISA

ID gène: 5979

UniProt: P07949

Pathways: Signalisation RTK, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation