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ATF2 Kit ELISA

ATF2 Reactivité: Humain, Rat, Souris pSer386 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN4886273
  • Antigène Voir toutes ATF2 Kits ELISA
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Épitope
    pSer386
    Reactivité
    • 12
    • 9
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human, Mouse and Rat Phospho-RSK2 (Ser386) ELISA Kit. This assay semi-quantitatively measures phophorylated RSK2 (Ser386) in lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human, Mouse and Rat ATF2 phosphorylated at site Serine-386.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes ATF2 Kits ELISA
    ATF2 (Activating Transcription Factor 2 (ATF2))
    Autre désignation
    ATF2 (ATF2 Produits)
    Synonymes
    CRE-BP1 Kit ELISA, CREB2 Kit ELISA, HB16 Kit ELISA, TREB7 Kit ELISA, CG12850 Kit ELISA, CG3749 Kit ELISA, dATF-2 Kit ELISA, DmelCG30420 Kit ELISA, CG30420 Kit ELISA, CHN1 Kit ELISA, Atf-2 Kit ELISA, CRE-BP Kit ELISA, Creb2 Kit ELISA, D130078H02Rik Kit ELISA, D18875 Kit ELISA, Tg(Gzma-Klra1)7Wum Kit ELISA, mXBP Kit ELISA, atf2-A Kit ELISA, atf2l Kit ELISA, activating transcription factor 2 Kit ELISA, Activating transcription factor-2 Kit ELISA, activating transcription factor 2 S homeolog Kit ELISA, ATF2 Kit ELISA, Atf-2 Kit ELISA, Atf2 Kit ELISA, atf2.S Kit ELISA, atf2 Kit ELISA
    ID gène
    6197
    UniProt
    P51812, P18654, D3Z8E0
    Pathways
    Signalisation MAPK, Signalisation RTK, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
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