RPS6KA3
(Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))
Épitope
pSer386, total
Reactivité
Humain, Souris, Rat
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human, Mouse and Rat Phospho-RSK2 (Ser386) and Total RSK2 ELISA Kit. This assay semi-quantitatively measures phophorylated RSK2 (Ser386) and Total RSK2 in lysate samples.
Type d'échantillon
Cell Culture Lysate
Analytical Method
Semi-Quantitative
Specificité
This ELISA kit recognizes Human, Mouse and Rat RSK2 phosphorylated at site Serine-386 as well as total RSK2.
Attributs du produit
Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material
Ingrédients
Pre-Coated 96-well Strip Microplate
Wash Buffer
Anti-Phospho Antibody
Anti-Pan Antibody
HRP-Conjugated Secondary Antibody
Streptavidin-Conjugated HRP
Assay Diluent
TMB One-Step Substrate
Stop Solution
Lysis Buffer
Positive Control Sample
Matériel non inclus
Distilled or deionized water
100 mL and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions
For Research Use only
Stock
-20 °C
Stockage commentaire
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.