SMAD4 Kit ELISA

N° du produit ABIN4886294

Détail du produit SMAD4 Kit ELISA

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Épitope: pThr277, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-SMAD4 (Thr277) and Total SMAD4 ELISA Kit. This assay semi-quantitatively measures phophorylated SMAD4 (Thr277) and Total SMAD4 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human SMAD4 phosphorylated at site Threonine-277 as well as total SMAD4.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: SMAD4 (SMAD4 Produits)

Synonymes: smad4 Kit ELISA, DPC4 Kit ELISA, JIP Kit ELISA, MADH4 Kit ELISA, MYHRS Kit ELISA, AW743858 Kit ELISA, D18Wsu70e Kit ELISA, Madh4 Kit ELISA, XSmad4alpha Kit ELISA, Xsmad4 Kit ELISA, dpc4 Kit ELISA, jip Kit ELISA, madh4 Kit ELISA, xsmad4a Kit ELISA, SMAD family member 4 Kit ELISA, Smad4 protein Kit ELISA, SMAD family member 4, gene 1 L homeolog Kit ELISA, SMAD4 Kit ELISA, smad4 Kit ELISA, Smad4 Kit ELISA, smad4.1.L Kit ELISA

ID gène: 4089

UniProt: Q13485

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy