STAT5A Kit ELISA

N° du produit ABIN4889793

Détail du produit STAT5A Kit ELISA

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Épitope: pTyr694, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-STAT5A (Tyr694) and Total STAT5A ELISA Kit. This assay semi-quantitatively measures STAT5A phosphorylated at Tyrosine-694 as well as total STAT5A in cell lysate samples.

Type d'échantillon: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694 as well as total STAT5A.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Autre désignation: STAT5A (STAT5A Produits)

Synonymes: MGF Kit ELISA, STAT5 Kit ELISA, AA959963 Kit ELISA, Stat5 Kit ELISA, STAT5A/MGF Kit ELISA, STATA5 Kit ELISA, STAT5A Kit ELISA, stat5 Kit ELISA, stat5b Kit ELISA, signal transducer and activator of transcription 5A Kit ELISA, signal transducer and activator of transcription 5a Kit ELISA, STAT5A Kit ELISA, Stat5a Kit ELISA, stat5a Kit ELISA, LOC100348518 Kit ELISA, LOC100716811 Kit ELISA

ID gène: 6776

UniProt: P42229

Pathways: Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation