Cytochrome C Kit ELISA

N° du produit ABIN4986817

Détail du produit Cytochrome C Kit ELISA

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 39-2500 pg/mL

Seuil minimal de détection: 39 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant Human Cytochrome C Ligand

Sensibilité: 10 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome C (CYCS Produits)

Synonymes: CYC Kit ELISA, HCS Kit ELISA, THC4 Kit ELISA, cyct Kit ELISA, cyc Kit ELISA, hcs Kit ELISA, CYCS Kit ELISA, cytc Kit ELISA, CYCSA Kit ELISA, CG17903 Kit ELISA, Cyt C Kit ELISA, Cyt c Kit ELISA, Cyt-c2 Kit ELISA, CytC-2 Kit ELISA, Cytc Kit ELISA, Cytc-p Kit ELISA, DC4 Kit ELISA, DMc01 Kit ELISA, Dmel\\CG17903 Kit ELISA, cyt c Kit ELISA, cyt c-p Kit ELISA, cyt-c-p Kit ELISA, cyt-c2 Kit ELISA, cyt.c Kit ELISA, cytochrome c Kit ELISA, dc4 Kit ELISA, pDMc01 Kit ELISA, wu:fk52b01 Kit ELISA, zgc:86706 Kit ELISA, ENSMUSG00000058927 Kit ELISA, cytochrome c, somatic Kit ELISA, cytochrome c, somatic S homeolog Kit ELISA, Cytochrome c proximal Kit ELISA, cytochrome c, somatic b Kit ELISA, cytochrome c, somatic pseudogene Kit ELISA, cytochrome c-like Kit ELISA, cytochrome c Kit ELISA, CYCS Kit ELISA, cycs.S Kit ELISA, cycs Kit ELISA, Cycs Kit ELISA, Cyt-c-p Kit ELISA, cycsb Kit ELISA, Gm10053 Kit ELISA, LOC100724240 Kit ELISA, CYC Kit ELISA, LOC100847700 Kit ELISA, LOC101107954 Kit ELISA, LOC106829744 Kit ELISA, LOC106992390 Kit ELISA, LOC102085712 Kit ELISA

Sujet: Human somatic cytochrome c is a 15 kDa, 104 amino acid (aa) polypeptide that participates in both oxidative phosphorylation and apoptosis (1). It exhibits 91 % aa identity with mouse/rat cytochrome c (1-3). The molecule is initially synthesized in the cytoplasm as an extended, 12 kDa apoprotein. This molecule is subsequently transported across the outer mitochondrial membrane into the intermitochondrial space via a receptor-independent process. Here, it associates with an inner mitochondrial membrane enzyme called CCHL (cytochrome c heme lyase) which covalently attaches one heme molecule to the N-terminus, forming functional cytochrome c (4-6). This covalent attachment causes cytochrome c to undergo a conformational change to a globular molecule effectively trapping cytochrome c in the intermitochrondrial space.Within the intermitochondrial space, cytochrome c is actively involved in the oxidative phosphorylation pathway. It transports electrons from the cytochrome c reductase complex to the cytochrome c oxidase complex (7-9). This transports excess electrons along the respiratory pathway and generates ATP for energy-dependent processes. Alternatively, and in response to apoptotic signals, cytochrome c can be released from mitochondria into the cytosol. Here, it activates an apoptotic program via one of many possible caspase-driven cascades (8, 10-12). The events which trigger an apoptotic signal (such as growth factor withdrawal) are not well understood. The result of such a signal, however, has been suggested to be a translocation of cytosolic Bax and/or Bad to the outer mitochondrial membrane where they overcome a Bcl-2-mediated stabilization (13-15). Bax-mediated destabilization may take the form of channel creation or mitochondrial swelling, resulting in cytochrome c release (15-18). Whatever the mechanism, released cytochrome c participates in the formation of a cytosolic complex which is composed of 15 kDa cytochrome c, 130 kDa Apaf-1 (apoptotic protease activating factor-1), dATP, and 46 kDa Apaf-3/caspase-9 (15, 18, 19). Within this complex, Apaf-3/caspase-9 is activated, leading to the downstream activation of caspases-3,-7 and -9, followed by additional caspases that ultimately lead to cellular apoptosis (10, 18, 20,21).

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity