IL-22 Kit ELISA

N° du produit ABIN4986947

Détail du produit IL-22 Kit ELISA

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant Human IL-22 Ligand

Sensibilité: 15 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL-22 (IL22) (Interleukin 22 (IL22))

Autre désignation: IL-22 (IL22 Produits)

Sujet: Interleukin 22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF), is a member of the IL-10 cytokine family. Other members of this family include IL-10, IL-19, IL-20, IL-24, and IL-26 (1). IL-22 was initially identified as a gene induced by IL-9 in mouse T cells and mast cells (2). Human IL-22 cDNA encodes a 179 amino acid (aa) protein with a putative 33 aa signal peptide, sharing approximately 79 % and 22 % aa sequence identity with mouse IL-22 and human IL-10, respectively (3, 4). Although the related IL-10 is thought to act as a dimer, the crystal structure of IL-22 suggests it may interact with its receptor as a monomer (5). The functional IL-22 receptor is of the class 2 subtype and consists of two receptor subunits,IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R (previously known as CRF2-4) (6). The IL-10 R chain is shared by IL-10, IL-26, IL-28A, IL-28B, and IL-29 (7, 8).IL-22 R is expressed primarily in the pancreas, and to a lesser extent, tissues of the gastrointestinal tract, kidney, and skin (7, 9 - 12). A soluble receptor, IL-22 binding protein (IL-22BP), has also been described and may act as an endogenous inhibitor of IL-22 activity (13 - 15). IL-22 has been shown to activate Jak/STAT and MAPK signaling pathways and upregulate the production of acute phase proteins (3, 4, 6, 16 - 18).IL-22 is produced primarily by activated Th1-type T cells and NK cells (19). Mouse IL-22 expression is induced in various organs upon lipopolysaccharide injection, suggesting that it may be involved in inflammatory responses (3). In humans, this is supported by the observation that IL-22 is produced by synovial fibroblasts and macrophages of rheumatoid arthritis (RA) patients and is capable of inducing pro-inflammatory responses in RA synovial tissues (20). In addition, it stimulates the production of pro-inflammatory cytokines and anti-microbial defensins in human keratinocytes (9, 10). These activities result in epidermal hyperplasia in models of human skin (9).