Cyclin E1 Kit ELISA

N° du produit ABIN4993358

Détail du produit Cyclin E1 Kit ELISA

Antigène: Cyclin E1 (CCNE1)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125-5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCNE1 in Mouse serum, plasma and other biological fluids

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes natural and recombinantMouseCCNE1. No significant cross-reactivity or interference between MouseCCNE1 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseCCNE1 and all the analogues.

Sensibilité: 46.875 pg/mL

Ingrédients: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Matériel non inclus: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Information d'application

Commentaires:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Volume d'échantillon: 100 µL

Plaque: Pre-coated

Protocole: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CCNE1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CCNE1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CCNE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CCNE1.You can calculate the concentration of CCNE1in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Stock: 4 °C/-20 °C

Stockage commentaire: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Détail du antigène

Antigène: Cyclin E1 (CCNE1)

Autre désignation: Cyclin-E1 (CCNE1) (CCNE1 Produits)

Synonymes: CCNE1 Kit ELISA, ccne Kit ELISA, cyclinE Kit ELISA, CCNE Kit ELISA, AW538188 Kit ELISA, CycE1 Kit ELISA, CYCLE Kit ELISA, Ccne Kit ELISA, cyce2 Kit ELISA, cycE3 Kit ELISA, cyce Kit ELISA, fa18e07 Kit ELISA, wu:fa18e07 Kit ELISA, cyclin E1 Kit ELISA, cyclin E1 L homeolog Kit ELISA, cyclin E1 S homeolog Kit ELISA, CCNE1 Kit ELISA, ccne1 Kit ELISA, Ccne1 Kit ELISA, ccne1.L Kit ELISA, ccne1.S Kit ELISA

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases