CDK4 Kit ELISA

N° du produit ABIN4993370

Détail du produit CDK4 Kit ELISA

Antigène: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDK4 in Mouse serum, plasma and other biological fluids

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes natural and recombinantMouseCDK4. No significant cross-reactivity or interference between MouseCDK4 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseCDK4 and all the analogues.

Sensibilité: 37.5 pg/mL

Ingrédients: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Matériel non inclus: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Information d'application

Commentaires:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Volume d'échantillon: 100 µL

Plaque: Pre-coated

Protocole: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CDK4.You can calculate the concentration of CDK4in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Stock: 4 °C/-20 °C

Stockage commentaire: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Détail du antigène

Antigène: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Autre désignation: Cyclin Dependent Kinase 4 (CDK4) (CDK4 Produits)

Synonymes: zgc:153726 Kit ELISA, CMM3 Kit ELISA, PSK-J3 Kit ELISA, Crk3 Kit ELISA, 8-6 Kit ELISA, CDK4 Kit ELISA, CDK4/6 Kit ELISA, CG5072 Kit ELISA, Cdk4/6 Kit ELISA, DmCdk4 Kit ELISA, Dmel\\CG5072 Kit ELISA, Pk53C Kit ELISA, Pk?7 Kit ELISA, cdk Kit ELISA, cdk4 Kit ELISA, cdk4/6 Kit ELISA, dCdk4 Kit ELISA, l(2)05428 Kit ELISA, l(2)0671 Kit ELISA, l(2)k06503 Kit ELISA, l(2)s4639 Kit ELISA, l(2)sh0671 Kit ELISA, xcdk4 Kit ELISA, cyclin-dependent kinase 4 Kit ELISA, cyclin dependent kinase 4 Kit ELISA, Cyclin-dependent kinase 4 Kit ELISA, cyclin-dependent kinase 4 L homeolog Kit ELISA, cdk4 Kit ELISA, CDK4 Kit ELISA, Cdk4 Kit ELISA, cdk4.L Kit ELISA

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, Regulation of Cell Size