Dihydrofolate Reductase Kit ELISA
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- Antigène Voir toutes Dihydrofolate Reductase (DHFR) Kits ELISA
- Dihydrofolate Reductase (DHFR)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.125-5000 pg/mL
- Seuil minimal de détection
- 78.125 pg/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DHFR in human serum, plasma and other biological fluids
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This kit recognizes natural and recombinantHumanDHFR. No significant cross-reactivity or interference between HumanDHFR and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanDHFR and all the analogues.
- Sensibilité
- 46.875 pg/mL
- Ingrédients
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Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis - Matériel non inclus
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Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer - Featured
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- Commentaires
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Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - Volume d'échantillon
- 100 µL
- Plaque
- Pre-coated
- Protocole
- This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to DHFR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for DHFRand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain DHFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of DHFR.You can calculate the concentration of DHFRin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- Restrictions
- For Research Use only
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- Conseil sur la manipulation
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
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- Antigène Voir toutes Dihydrofolate Reductase (DHFR) Kits ELISA
- Dihydrofolate Reductase (DHFR)
- Abstract
- DHFR Produits
- Synonymes
- DHFR Kit ELISA, F10_orf160 Kit ELISA, ECK0049 Kit ELISA, JW0047 Kit ELISA, tmrA Kit ELISA, cb595 Kit ELISA, zgc:86637 Kit ELISA, DDBDRAFT_0187132 Kit ELISA, DDBDRAFT_0230113 Kit ELISA, DDB_0187132 Kit ELISA, DDB_0230113 Kit ELISA, CG14887 Kit ELISA, Dmel\\CG14887 Kit ELISA, dhfr Kit ELISA, DHFRP1 Kit ELISA, DYR Kit ELISA, 8430436I03Rik Kit ELISA, AA607882 Kit ELISA, AI662710 Kit ELISA, AW555094 Kit ELISA, Dhfr1 Kit ELISA, confers resistance to sulfonamides Kit ELISA, dihydrofolate reductase Kit ELISA, dihydrofolate reductase DhfR Kit ELISA, Dihydrofolate reductase Kit ELISA, dhfr Kit ELISA, DHFR Kit ELISA, dhfR Kit ELISA, folA Kit ELISA, dfrA Kit ELISA, LOC707382 Kit ELISA, CNJ01940 Kit ELISA, DDB_G0286755 Kit ELISA, Dhfr Kit ELISA, Mesil_0096 Kit ELISA, Arch_0408 Kit ELISA, Fbal_3367 Kit ELISA, Ilyop_0482 Kit ELISA, Lbys_2007 Kit ELISA, Riean_0733 Kit ELISA, Mrub_1855 Kit ELISA, Poras_0766 Kit ELISA, Halhy_3552 Kit ELISA, Mesop_3614 Kit ELISA, LOC609048 Kit ELISA
- Pathways
- Mitotic G1-G1/S Phases
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