MIF Kit ELISA

N° du produit ABIN5026922

Détail du produit MIF Kit ELISA

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781-50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human MIF concentrations in serum, plasma and other biological fluids.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Sensibilité: < 0.469 ng/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Autre désignation: MIF (Macrophage migration inhibitory factor) (MIF Produits)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling