IL-7 Kit ELISA

N° du produit ABIN5026943

Détail du produit IL-7 Kit ELISA

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8-500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human IL-7 concentrations in serum, plasma and other biological fluids.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Sensibilité: < 4.69 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-humanIL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-7antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Autre désignation: IL-7 (Interleukin 7) (IL7 Produits)

Pathways: Signalistation JAK/STAT