CCL3 Kit ELISA
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- Antigène Voir toutes CCL3 Kits ELISA
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 23.44-1500 pg/mL
- Seuil minimal de détection
- 23.44 pg/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human MIP-1Alpha /CCL3 concentrations in serum, plasma and other biological fluids.
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensibilité
- < 14 pg/mL
- Ingrédients
- plate, standard, diluent, antibodies
- Matériel non inclus
- pipettes, tubes, reader, incubator
- Top Product
- Discover our top product CCL3 Kit ELISA
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Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 Reactivité: Souris Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Chien Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Souris Colorimetric Sandwich ELISA 8-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Souris AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Rat AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Souris Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Humain Colorimetric Sandwich ELISA 3.12-200 pg/mL Plasma, Serum, Tissue Homogenate
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3 - 4 h
- Plaque
- Pre-coated
- Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MIP-1Alpha /CCL3 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MIP-1Alpha /CCL3 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1Alpha /CCL3 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MIP-1Alpha /CCL3 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Précaution d'utilisation
- protect your eyes
- Stock
- 4 °C
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- Antigène Voir toutes CCL3 Kits ELISA
- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
- Autre désignation
- MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha) (CCL3 Produits)
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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