Bcl-2 Kit ELISA
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- Antigène Voir toutes Bcl-2 (BCL2) Kits ELISA
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156-10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human Bcl-2 concentrations in serum, plasma and other biological fluids.
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensibilité
- < 0.094 ng/mL
- Ingrédients
- plate, standard, diluent, antibodies
- Matériel non inclus
- pipettes, tubes, reader, incubator
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- Discover our best selling BCL2 Kit ELISA
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- Discover our top product BCL2 Kit ELISA
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3 - 4 h
- Plaque
- Pre-coated
- Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Bcl-2 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human Bcl-2 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Bcl-2 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human Bcl-2 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Précaution d'utilisation
- protect your eyes
- Stock
- 4 °C
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- Antigène Voir toutes Bcl-2 (BCL2) Kits ELISA
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Autre désignation
- Bcl -2 (B cell Lymphoma 2) (BCL2 Produits)
- Synonymes
- Bcl-2 Kit ELISA, PPP1R50 Kit ELISA, bcl-2 Kit ELISA, AW986256 Kit ELISA, C430015F12Rik Kit ELISA, D630044D05Rik Kit ELISA, D830018M01Rik Kit ELISA, BCL-2 Kit ELISA, Bcl2 Kit ELISA, BCL2, apoptosis regulator Kit ELISA, B cell leukemia/lymphoma 2 Kit ELISA, B-cell CLL/lymphoma 2a Kit ELISA, B-cell CLL/lymphoma 2 protein Kit ELISA, BCL2 Kit ELISA, Bcl2 Kit ELISA, bcl2a Kit ELISA, BCL-2 Kit ELISA
- Pathways
- Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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