IL-13 Kit ELISA
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- Antigène Voir toutes IL-13 (IL13) Kits ELISA
- IL-13 (IL13) (Interleukin 13 (IL13))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6-1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-13 concentrations in serum, plasma and other biological fluids.
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensibilité
- < 9.4 pg/mL
- Ingrédients
- plate, standard, diluent, antibodies
- Matériel non inclus
- pipettes, tubes, reader, incubator
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- Discover our best selling IL13 Kit ELISA
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- Discover our top product IL13 Kit ELISA
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3 - 4 h
- Plaque
- Pre-coated
- Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-13 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-13 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-13 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-13 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Précaution d'utilisation
- protect your eyes
- Stock
- 4 °C
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- Antigène Voir toutes IL-13 (IL13) Kits ELISA
- IL-13 (IL13) (Interleukin 13 (IL13))
- Autre désignation
- IL-13 (Interleukin 13) (IL13 Produits)
- Synonymes
- IL13 Kit ELISA, IL-13 Kit ELISA, P600 Kit ELISA, Il-13 Kit ELISA, L13 Kit ELISA, interleukin 13 Kit ELISA, IL13 Kit ELISA, Il13 Kit ELISA
- Pathways
- Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
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