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Phenylalanine Hydroxylase Kit ELISA

Reactivité: Chemical Colorimetric Sandwich ELISA Plasma, Serum, Tissue Homogenate
N° du produit ABIN5067907
  • Antigène Voir toutes Phenylalanine Hydroxylase Kits ELISA
    Phenylalanine Hydroxylase
    Reactivité
    • 5
    • 3
    • 1
    • 1
    • 1
    Chemical
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    PAH (Phenylalanine Hydroxylase) ELISA Kit is a quantitative sandwich assay for the detection of PAH in human serum, plasma, tissue homogenates and other biological fluids
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    46.875 pg/mL
    Attributs du produit
    PAH (Phenylalanine Hydroxylase) ELISA Kit (Human)
    Ingrédients
    • *357883A: Microtiter Strips, 1x96 wells (8x12 wells).
    • *357883B: Standard, 2x1 vial
    • 357883C: Sample/Standard Dilution Buffer, 1x20ml
    • 357883D: Antibody (Biotin) (Concentrated), 1x120ul
    • 357883E: Antibody Dilution Buffer, 1x10ml
    • 357883F: Streptavidin (HRP) (SABC), 1x120ul
    • 357883G: SABC Dilution Buffer, 1x10ml
    • 357883H: TMB Substrate, 1x10ml
    • 357883J: Stop Solution, 1x10ml
    • 357883K: Wash Buffer, 25X, 1x30ml
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  • Plaque
    Pre-coated
    Protocole
    Principle:
    • This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to PAH. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain PAH, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAH in the sample is then determined by comparing the O.D. of the sample to the standard curve.
    Précision du teste
    Precision:
    • Intra-Assay CV: <8%
    • Inter-Assay CV: <10%
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    4°C/-20°C
  • Antigène Voir toutes Phenylalanine Hydroxylase Kits ELISA
    Phenylalanine Hydroxylase
    Autre désignation
    PAH (Phenylalanine Hydroxylase) (Phenylalanine Hydroxylase Produits)
    Synonymes
    cb877 Kit ELISA, wu:fb02f10 Kit ELISA, zgc:65981 Kit ELISA, GB18494 Kit ELISA, PAH Kit ELISA, pku Kit ELISA, pku1 Kit ELISA, DDBDRAFT_0206188 Kit ELISA, DDBDRAFT_0231664 Kit ELISA, DDB_0206188 Kit ELISA, DDB_0231664 Kit ELISA, AW106920 Kit ELISA, PH Kit ELISA, PKU Kit ELISA, PKU1 Kit ELISA, phenylalanine-4-hydroxylase Kit ELISA, phenylalanine hydroxylase Kit ELISA, protein henna Kit ELISA, phenylalanine 4-monooxygenase Kit ELISA, pah Kit ELISA, LOC408622 Kit ELISA, PAH Kit ELISA, CpipJ_CPIJ002149 Kit ELISA, Pah Kit ELISA
    Classe de substances
    Chemical
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