Defensin beta 3 Kit ELISA
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- Antigène Voir toutes Defensin beta 3 (DEFB3) Kits ELISA
- Defensin beta 3 (DEFB3)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 12.5-400 pg/mL
- Seuil minimal de détection
- 12.5 pg/mL
- Application
- ELISA
- Fonction
- The Human Beta Defensin 3 (BD-3) ELISA Kit is an in vitro immunoassay for research use in the quantification of BD-3 in cultures of human cells and in appropriately qualified samples from serum, saliva, or other tissue fluids. The kit is suitable for testing a variety of sample types, in accordance with appropriate validation of linearity and recovery.
- Type d'échantillon
- Serum, Cell Culture Samples, Saliva, Tissue Samples
- Analytical Method
- Quantitative
- Sensibilité
- 20 pg/mL
- Attributs du produit
- Beta Defensin 3, Human, ELISA Kit (BD3)
- Ingrédients
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- Microwell Strips, 1x96 wells (12x8-well strips). Coated with purified anti-human BD3 antibodies. Return unused strips to the pouch with desiccant, re-seal and store refrigerated.
- Standard, 400pg/ml, 2x1 vial. Recombinant human BD3. Supplied as a lyophilized powder from buffer, protein stabilizers.
- Ab (Biotin), 100X, 1x150ul. Biotinylated anti-human BD3. Supplied as a liquid in buffer, protein, detergents, 0.05% Bronidox.
- Streptavidin (HRP), 100X, 1x150ul. Supplied as a liquid in buffer with protein, detergents, 0.05% Bronidox.
- Sample Diluent, 10X, 1x10ml.
- Biotin, HRP Diluent, 1x22ml. Supplied as a liquid in buffer, protein, anti-microbials.
- Wash Solution, 100X, 1x10ml.
- TMB Solution, 1x12ml. Chromogenic substrate for HRP containing TMB and peroxide.
- Stop Solution (1% O4), 1x12ml. 1% sulfuric acid. Caution: Corrosive!
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- Plaque
- Pre-coated
- Protocole
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Principle:
- The assay is based on the binding of human Beta Defensin 3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to biotin, which then binds to a streptavidin (HRP). After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of BD-3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of BD-3 in samples is calculated from a standard curve of purified recombinant human BD-3 of designated concentration.
- Restrictions
- For Research Use only
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- Stock
- 4 °C
- Stockage commentaire
- 4°C
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- Antigène Voir toutes Defensin beta 3 (DEFB3) Kits ELISA
- Defensin beta 3 (DEFB3)
- Autre désignation
- Beta Defensin 3 (DEFB3 Produits)
- Synonymes
- BD-3 Kit ELISA, DEFB-3 Kit ELISA, DEFB103 Kit ELISA, DEFB3 Kit ELISA, HBD3 Kit ELISA, HBP-3 Kit ELISA, HBP3 Kit ELISA, hBD-3 Kit ELISA, BNBD-3 Kit ELISA, BNBD-6 Kit ELISA, BNDB-3 Kit ELISA, BNDB-6 Kit ELISA, BNDB3 Kit ELISA, DEFB103A Kit ELISA, DEFB2 Kit ELISA, DEFB6 Kit ELISA, bnbd3 Kit ELISA, defb405 Kit ELISA, defensin beta 103A Kit ELISA, defensin beta 3 Kit ELISA, beta-defensin 3 Kit ELISA, DEFB103A Kit ELISA, Defb3 Kit ELISA, DEFB3 Kit ELISA
- Pathways
- Production of Molecular Mediator of Immune Response
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