Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Mesothelin Kit ELISA

MSLN Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Lysate, Plasma, Serum
N° du produit ABIN5068269
  • Antigène Voir toutes Mesothelin (MSLN) Kits ELISA
    Mesothelin (MSLN)
    Reactivité
    • 9
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Type d'échantillon
    Cell Culture Lysate, Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    0.010 ng/mL
    Attributs du produit
    Mesothelin, ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)
    Ingrédients
    • Mesothelin Microplate: 12 strips x 8 wells
    • Mesothelin monoclonal antibody, conjugated to HRP: 1 x 21ml
    • Mesothelin Standard (lyophilized): 1 x 100ng
    • Assay Diluent R 1 x 11ml
    • Calibrator Diluent R Concentrate: 1 x 21ml
    • Wash Buffer Concentrate: 1 x 21ml
    • Color Reagent A (stabilized hydrogen peroxide): 1 x 12.5ml
    • Color Reagent B (tetramethylbenzidine): 1 x 12.5ml
    • Stop Solution (2N sulfuric acid): 1 x 6ml
    • Plate Covers: 4 adhesive strips
    Featured
    Discover our best selling MSLN Kit ELISA
    Top Product
    Discover our top product MSLN Kit ELISA
  • Plaque
    Pre-coated
    Protocole
    Principle:
    • This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mesothelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Mesothelin present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for Mesothelin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Stockage commentaire
    4°C
  • Antigène Voir toutes Mesothelin (MSLN) Kits ELISA
    Mesothelin (MSLN)
    Autre désignation
    Mesothelin (MSLN Produits)
    Synonymes
    MPF Kit ELISA, SMRP Kit ELISA, MSLN Kit ELISA, mesothelin Kit ELISA, MSLN Kit ELISA, Msln Kit ELISA, LOC611363 Kit ELISA, LOC100524016 Kit ELISA
    Sujet
    Mesothelin, also known as CAK1 and ERC, is a protein that is mainly expressed in the mesothelial cells lining the pleura, pericardium, and peritoneum. The Mesothelin gene encodes a precursor protein of 70kD that is cleaved into two products, including megakaryocyte potentiating factor (aa37-286) and Mesothelin (aa296-606). Megakaryocyte potentiation factor is a secreted protein of about 32kD, and it functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes.
    Pathways
    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
Vous êtes ici:
Support technique