NOS2 Kit ELISA

N° du produit ABIN5526682

Détail du produit NOS2 Kit ELISA

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Épitope: phosphorylated

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human, Mouse and Rat Phosphotyrosine iNOS ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated iNOS in cell lysate samples.

Type d'échantillon: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes human iNOS phosphotyrosine.

Attributs du produit:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Autre désignation: iNOS (NOS2 Produits)

Synonymes: HEP-NOS Kit ELISA, INOS Kit ELISA, NOS Kit ELISA, NOS2A Kit ELISA, NOS-II Kit ELISA, Nos-2 Kit ELISA, Nos2a Kit ELISA, i-NOS Kit ELISA, iNOS Kit ELISA, iNos Kit ELISA, NOS2a Kit ELISA, NOS2 Kit ELISA, BmNOS2 Kit ELISA, Nsl Kit ELISA, nos2 Kit ELISA, nitric oxide synthase 2 Kit ELISA, nitric oxide synthase 2, inducible Kit ELISA, inducible nitric oxide synthase Kit ELISA, inducible nitric-oxide synthase Kit ELISA, nanos C2HC-type zinc finger 2 Kit ELISA, NOS2 Kit ELISA, Nos2 Kit ELISA, nos2 Kit ELISA, LOC396821 Kit ELISA, NANOS2 Kit ELISA

ID gène: 4843

UniProt: P35228, P29477, Q06518

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process