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Pro-Cathepsin B Kit ELISA

Reactivité: Humain Colorimetric Sandwich ELISA 61 pg/mL-15 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN5526685
  • Antigène Tous les produits Pro-Cathepsin B
    Pro-Cathepsin B
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    61 pg/mL-15 ng/mL
    Seuil minimal de détection
    61 ng/mL
    Application
    ELISA
    Fonction
    Human Pro-Cathepsin B ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Pro-Cathepsin B.
    Sensibilité
    61 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Préparation des réactifs

    Recommended Dilution for serum and plasma samples4 fold

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Date de péremption
    6 months
  • Antigène Tous les produits Pro-Cathepsin B
    Pro-Cathepsin B
    Abstract
    Pro-Cathepsin B Produits
    ID gène
    1508
    UniProt
    P07858
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