eIF4EBP1 Kit ELISA

N° du produit ABIN5526687

Détail du produit eIF4EBP1 Kit ELISA

Antigène: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Épitope: pThr36, total

Reactivité: Souris, Humain

Méthode de détection: Colorimetric

Type de méthode: Cell ELISA

Application: ELISA

Fonction: Human and Mouse Phospho-4E-BP1 (Thr36) and Total 4E-BP1 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures 4E-BP1 phosphorylated at Threonine-36 as well as total 4E-BP1 in adherent cell lines.

Type d'échantillon: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human and Mouse 4E-BP1 phosphorylated at site Threonine-36 as well as total 4E-BP1.

Attributs du produit:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Ingrédients:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Autre désignation: 4E-BP1 (EIF4EBP1 Produits)

Synonymes: 4E-BP1 Kit ELISA, 4EBP1 Kit ELISA, BP-1 Kit ELISA, PHAS-I Kit ELISA, 4e-bp1 Kit ELISA, AA959816 Kit ELISA, 153432_at Kit ELISA, 43-BP Kit ELISA, 4E-BP Kit ELISA, 4EBP Kit ELISA, 4e-bp Kit ELISA, 4eBP Kit ELISA, 4ebp Kit ELISA, BcDNA.HL08053 Kit ELISA, BcDNA:HL08053 Kit ELISA, CG8846 Kit ELISA, CT8705 Kit ELISA, Dm 4E-BP Kit ELISA, Dmel\\CG8846 Kit ELISA, Phas1 Kit ELISA, Thor/EIF4EBP Kit ELISA, anon-WO0172774.158 Kit ELISA, d4E-BP Kit ELISA, d4E-BP1 Kit ELISA, d4EBP Kit ELISA, d4e-bp Kit ELISA, d4eBP Kit ELISA, dthor Kit ELISA, eIF4E-BP Kit ELISA, l(2)06270 Kit ELISA, l(2)k07736 Kit ELISA, l(2)k13517 Kit ELISA, pp20 Kit ELISA, thor Kit ELISA, EIF4EBP1 Kit ELISA, fc04h09 Kit ELISA, wu:fc04h09 Kit ELISA, zgc:64137 Kit ELISA, eukaryotic translation initiation factor 4E binding protein 1 Kit ELISA, CG8846 gene product from transcript CG8846-RA Kit ELISA, EIF4EBP1 Kit ELISA, Eif4ebp1 Kit ELISA, Thor Kit ELISA, eif4ebp1 Kit ELISA

ID gène: 1978

UniProt: Q13541, Q60876

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, BCR Signaling