CREB1 Kit ELISA

N° du produit ABIN5526692

Détail du produit CREB1 Kit ELISA

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Épitope: phosphorylated

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human pCREB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical CREB binding sequences phosphorylated at site serine residue 133 to semi-quantitatively detect active pCREB in lysates or nuclear extracts. Only available in North America.

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes human phosphorylated CREB in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Autre désignation: CREB (CREB1 Produits)

Synonymes: creb Kit ELISA, CREB1 Kit ELISA, Creb1 Kit ELISA, CREB Kit ELISA, 2310001E10Rik Kit ELISA, 3526402H21Rik Kit ELISA, AV083133 Kit ELISA, Creb Kit ELISA, Creb-1 Kit ELISA, creb1 Kit ELISA, zgc:55598 Kit ELISA, cAMP responsive element binding protein 1 Kit ELISA, cAMP responsive element binding protein Kit ELISA, cAMP responsive element binding protein 1 S homeolog Kit ELISA, cAMP responsive element binding protein 1a Kit ELISA, CREB1 Kit ELISA, creb1 Kit ELISA, CREB Kit ELISA, Creb1 Kit ELISA, creb1.S Kit ELISA, creb1a Kit ELISA

ID gène: 1385

UniProt: P16220

Pathways: Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation