GATA2 Kit ELISA

N° du produit ABIN5526693

Détail du produit GATA2 Kit ELISA

Antigène: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human GATA-2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA-2 binding sequences to semi-quantitatively detect active GATA-2 in lysates or nuclear extracts. Only available in North America.

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes human FRA-2 in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: GATA2 (GATA Binding Protein 2 (GATA2))

Autre désignation: GATA-2 (GATA2 Produits)

Synonymes: DCML Kit ELISA, MONOMAC Kit ELISA, NFE1B Kit ELISA, Gata-2 Kit ELISA, xgata-2 Kit ELISA, xgata2 Kit ELISA, GATA-2 Kit ELISA, NF-E1b Kit ELISA, GATA2 Kit ELISA, Gata2 Kit ELISA, gata2 Kit ELISA, zg2 Kit ELISA, zgc:91840 Kit ELISA, GATA binding protein 2 Kit ELISA, GATA binding protein 2 L homeolog Kit ELISA, amidase Kit ELISA, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A Kit ELISA, hypothetical protein Kit ELISA, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) Kit ELISA, GATA2 transcription factor Kit ELISA, putative gata binding factor Kit ELISA, GATA binding protein 2a Kit ELISA, GATA binding protein 2b Kit ELISA, GATA2 Kit ELISA, Gata2 Kit ELISA, gata2.L Kit ELISA, RR_RS20245 Kit ELISA, gatA2 Kit ELISA, Smp_018590 Kit ELISA, gata2a Kit ELISA, gata2b Kit ELISA

ID gène: 2624

UniProt: P23769

Pathways: Stem Cell Maintenance