TFEB Kit ELISA

N° du produit ABIN5526704

Détail du produit TFEB Kit ELISA

Antigène: TFEB (Transcription Factor EB (TFEB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human TFEB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical TFEB binding sequences to semi-quantitatively detect active TFEB in lysates or nuclear extracts. Only available in North America.

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Références pour TFEB Kit ELISA (ABIN5526704)

Mlejnek, Havlasek, Pastvova, Dolezel, Dostalova: "Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration." dans: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 153, pp. 113328, (2022) (PubMed).

Kim, Kim, Kim, Kim, Han, Byeon, Lee, Kim: "HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer." dans: Autophagy, Vol. 14, Issue 3, pp. 385-403, (2019) (PubMed).

Détail du antigène

Antigène: TFEB (Transcription Factor EB (TFEB))

Autre désignation: TFEB (TFEB Produits)

Synonymes: TFEB Kit ELISA, tcfeb Kit ELISA, MGC145796 Kit ELISA, alphatfeb Kit ELISA, ALPHATFEB Kit ELISA, BHLHE35 Kit ELISA, TCFEB Kit ELISA, Tcfeb Kit ELISA, bHLHe35 Kit ELISA, transcription factor EB Kit ELISA, TFEB Kit ELISA, tfeb Kit ELISA, Tfeb Kit ELISA

ID gène: 7942

UniProt: P19484