FOXO3 Kit ELISA

N° du produit ABIN5526709

Détail du produit FOXO3 Kit ELISA

Antigène: FOXO3 (Forkhead Box O3 (FOXO3))

Épitope: pSer263

Reactivité: Humain, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human and Mouse Phospho-FOXO3 (Ser253) ELISA Kit. This assay semi-quantitatively measures FOXO3 phosphorylated at Serine-263 in cell lysate samples.

Type d'échantillon: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human and Mouse FOXO3 phosphorylated at site Serine-263.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: FOXO3 (Forkhead Box O3 (FOXO3))

Autre désignation: FOXO3 (FOXO3 Produits)

Synonymes: AF6q21 Kit ELISA, FKHRL1 Kit ELISA, FKHRL1P2 Kit ELISA, FOXO2 Kit ELISA, FOXO3A Kit ELISA, zgc:92176 Kit ELISA, wu:fb50g02 Kit ELISA, wu:fi33f07 Kit ELISA, Fkhrl1 Kit ELISA, Foxo3a Kit ELISA, FoxO3A Kit ELISA, 1110048B16Rik Kit ELISA, 2010203A17Rik Kit ELISA, C76856 Kit ELISA, Fkhr2 Kit ELISA, FOXO-3 Kit ELISA, FOXO3 Kit ELISA, foxo3a Kit ELISA, xfoxo3 Kit ELISA, foxO3 Kit ELISA, foxO Kit ELISA, fkhr Kit ELISA, foxo5 Kit ELISA, wu:fj65a12 Kit ELISA, zFkhr Kit ELISA, forkhead box O3 Kit ELISA, forkhead box O3A Kit ELISA, forkhead box O3 L homeolog Kit ELISA, forkhead box O3b Kit ELISA, FOXO3 Kit ELISA, foxo3a Kit ELISA, Foxo3 Kit ELISA, foxo3.L Kit ELISA, foxo3 Kit ELISA, foxO3 Kit ELISA, foxo3b Kit ELISA

ID gène: 2309

UniProt: O43524, Q9WVH4

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis