PDYN Kit ELISA

N° du produit ABIN5526715

Détail du produit PDYN Kit ELISA

Antigène: PDYN (Prodynorphin (PDYN))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1-1.000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human/Mouse/Rat Dynorphin A EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Dynorphin

Sensibilité: 0.3 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samplesHuman: 8x / Mouse: 8x / Rat: 8x

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Références pour PDYN Kit ELISA (ABIN5526715)

Qi, Liu, Yi, Wu, Liu, Zhao, Shi, Ma, Wu: "Warming Moxibustion Relieves Chronic Visceral Hyperalgesia in Rats: Relations to Spinal Dynorphin and Orphanin-FQ System." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 920675, (2013) (PubMed).

Détail du antigène

Antigène: PDYN (Prodynorphin (PDYN))

Autre désignation: Dynorphin A (PDYN Produits)

Synonymes: pdyn Kit ELISA, ADCA Kit ELISA, PENKB Kit ELISA, SCA23 Kit ELISA, Dyn Kit ELISA, OLPB Kit ELISA, olpa Kit ELISA, XOLPA Kit ELISA, penkb Kit ELISA, Xen-dorphin Kit ELISA, olpb Kit ELISA, XOLPB Kit ELISA, wu:fj38c03 Kit ELISA, PDYN Kit ELISA, prodynorphin Kit ELISA, prodynorphin L homeolog Kit ELISA, prodynorphin S homeolog Kit ELISA, pdyn Kit ELISA, PDYN Kit ELISA, Pdyn Kit ELISA, pdyn.L Kit ELISA, pdyn.S Kit ELISA

ID gène: 5173

UniProt: P01213