PRKAA1 Kit ELISA

N° du produit ABIN5526722

Détail du produit PRKAA1 Kit ELISA

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Épitope: pSer487, total

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human, Mouse and Rat Phospho-AMPKa1 (Ser487) and Total AMPKa1 ELISA Kit. This assay semi-quantitatively measures AMPKa1 phosphorylated at Serine-487 as well as total AMPKa1 in cell lysate samples.

Type d'échantillon: Cell Culture Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human, Mouse and Rat AMPKa1 phosphorylated at site Serine-487 as well as total AMPKa1.

Attributs du produit:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Autre désignation: AMPKa1 (PRKAA1 Produits)

Synonymes: AMPK Kit ELISA, AMPKa1 Kit ELISA, cb116 Kit ELISA, im:7154392 Kit ELISA, sb:cb130 Kit ELISA, wu:fa94c10 Kit ELISA, AMPK1 Kit ELISA, AMPKalpha1 Kit ELISA, AI194361 Kit ELISA, AI450832 Kit ELISA, AL024255 Kit ELISA, C130083N04Rik Kit ELISA, ampk Kit ELISA, ampka1 Kit ELISA, PRKAA1 Kit ELISA, protein kinase AMP-activated catalytic subunit alpha 1 Kit ELISA, protein kinase, AMP-activated, alpha 1 catalytic subunit Kit ELISA, protein kinase, AMP-activated, alpha 1 catalytic subunit L homeolog Kit ELISA, PRKAA1 Kit ELISA, prkaa1 Kit ELISA, Prkaa1 Kit ELISA, prkaa1.L Kit ELISA

ID gène: 5562

UniProt: Q13131, Q5EG47, P54645

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, L'effet Warburg