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CDK1 Kit ELISA
CDK1
Reactivité: Humain
pThr161
Colorimetric
Cell ELISA
Adherent Cell Culture
N° du produit ABIN5526724
Détail du produit CDK1 Kit ELISA
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Antigène
Voir toutes CDK1 Kits ELISA
CDK1
(Cyclin-Dependent Kinase 1 (CDK1))
Épitope
pThr161, total
Reactivité
Toutes les réactivités sur CDK1 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Cell ELISA
Application
ELISA
Fonction
Cell-Based ELISA Kit. This assay semi-quantitatively measures CDK1 phosphorylated at Threonine-161 as well as total CDK1 in adherent cell lines.
Type d'échantillon
Adherent Cell Culture
Analytical Method
Semi-Quantitative
Specificité
This ELISA kit recognizes Human CDK1 phosphorylated at site Threonine-161 as well as total CDK1.
Attributs du produit
Rapidly measure phosphorylated protein in adherent cell lines Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose) No sample lysis is needed Compatible with a standard ELISA plate reader
Ingrédients
Uncoated 96-well Strip Microplate Wash Buffers Fixing Solution Quenching Buffer Blocking Buffer Anti-phospho antibody Anti-pan antibody HRP-Conjugated Secondary Antibody TMB One-Step Substrate Stop Solution
Matériel non inclus
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Plaque
Pre-coated
Protocole
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CDK1 Kits ELISA
CDK1
(Cyclin-Dependent Kinase 1 (CDK1))
Autre désignation
CDK1 (CDK1 Produits )
Synonymes
CDC2 Kit ELISA, CDC28A Kit ELISA, P34CDC2 Kit ELISA, Cdc2 Kit ELISA, Cdc2a Kit ELISA, p34 Kit ELISA, cdc2 Kit ELISA, wu:fc30e01 Kit ELISA, zgc:92032 Kit ELISA, PSTAIR Kit ELISA, cdc-2 Kit ELISA, cdc2-a Kit ELISA, cdc28a Kit ELISA, cdc2x1.1 Kit ELISA, p34cdc2 Kit ELISA, xcdc2 Kit ELISA, 5363 Kit ELISA, CDCDm Kit ELISA, CDK1 Kit ELISA, CDK1/CDC2 Kit ELISA, CG5363 Kit ELISA, Cdk-1 Kit ELISA, Cdk1 Kit ELISA, Dcdc2 Kit ELISA, Dm cdc2 Kit ELISA, DmCdc2 Kit ELISA, DmCdk1 Kit ELISA, Dmcdc2 Kit ELISA, Dmel\\CG5363 Kit ELISA, cdc Kit ELISA, cdc2Dm Kit ELISA, cdk1 Kit ELISA, dCdk1 Kit ELISA, group 4 Kit ELISA, l(2)31Eh Kit ELISA, CDC2A Kit ELISA, CDC2AAT Kit ELISA, CDK2 Kit ELISA, CDKA1 Kit ELISA, CDKA;1 Kit ELISA, CYCLIN-DEPENDENT KINASE A;1 Kit ELISA, cell division control 2 Kit ELISA, cdc2-b Kit ELISA, cdc2a Kit ELISA, cdc2x1.2 Kit ELISA, POL3 Kit ELISA, pold Kit ELISA, cyclin dependent kinase 1 Kit ELISA, cyclin dependent kinase like 1 Kit ELISA, Cell division control protein 2 1 Kit ELISA, cyclin-dependent kinase 1 Kit ELISA, cyclin-dependent kinase 1 S homeolog Kit ELISA, Cyclin-dependent kinase 1 Kit ELISA, cell division control 2 Kit ELISA, cell division control protein2 homolog Kit ELISA, cyclin-dependent kinase 1 L homeolog Kit ELISA, polymerase (DNA directed), delta 1, catalytic subunit L homeolog Kit ELISA, cyclin-dependent protein kinase Cdk1/Cdc2 Kit ELISA, CDK1 Kit ELISA, CDKL1 Kit ELISA, POPTR_0004s14080g Kit ELISA, Cdk1 Kit ELISA, cdk1 Kit ELISA, cdk1.S Kit ELISA, CDC2 Kit ELISA, cdc2 Kit ELISA, cdk-1 Kit ELISA, cdk1.L Kit ELISA, pold1.L Kit ELISA
ID gène
983
UniProt
P06493
Pathways
Cycle Cellulaire , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , Activation of Innate immune Response , Mitotic G1-G1/S Phases , DNA Replication , M Phase , Toll-Like Receptors Cascades , Synthesis of DNA
Vus récemment
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