NRF2 Kit ELISA

N° du produit ABIN5526731

Détail du produit NRF2 Kit ELISA

Antigène: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Mouse Nrf2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical Nrf2 binding sequences to semi-quantitatively detect active Nrf2 in lysates or nuclear extracts. Only available in North America.

Type d'échantillon: Cell Lysate, Nuclear Extract

Analytical Method: Semi-Quantitative

Specificité: The olionucleotide/antibody pair provided in this kit recognizes mouse Nrf2 in whole lysates and nuclear extracts.

Attributs du produit:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Ingrédients:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Autre désignation: Nrf2 (NFE2L2 Produits)

Synonymes: Nrf2 Kit ELISA, nfe2l2 Kit ELISA, wu:fc15g09 Kit ELISA, wu:fj67e03 Kit ELISA, NF-E2R2 Kit ELISA, NRF2 Kit ELISA, AI194320 Kit ELISA, nuclear factor, erythroid 2-like 2a Kit ELISA, nuclear factor, erythroid 2 like 2 S homeolog Kit ELISA, nuclear factor, erythroid 2 like 2 Kit ELISA, nuclear factor, erythroid derived 2, like 2 Kit ELISA, nuclear factor, erythroid 2-like 2 Kit ELISA, nfe2l2a Kit ELISA, nfe2l2.S Kit ELISA, NFE2L2 Kit ELISA, Nfe2l2 Kit ELISA

ID gène: 18024

UniProt: Q60795

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling