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SOX2 Kit ELISA

SOX2 Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
N° du produit ABIN5526732
  • Antigène Voir toutes SOX2 Kits ELISA
    SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
    Reactivité
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
    Human SOX2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical SOX2 binding sequences to semi-quantitatively detect active SOX2 in lysates or nuclear extracts. Only available in North America.
    Type d'échantillon
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificité
    The olionucleotide/antibody pair provided in this kit recognizes human SOX2 in whole lysates and nuclear extracts.
    Attributs du produit
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Ingrédients
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product SOX2 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes SOX2 Kits ELISA
    SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))
    Autre désignation
    SOX2 (SOX2 Produits)
    Synonymes
    ANOP3 Kit ELISA, MCOPS3 Kit ELISA, RGD1565646 Kit ELISA, Sox-2 Kit ELISA, lcc Kit ELISA, ysb Kit ELISA, cb236 Kit ELISA, wu:fb83g04 Kit ELISA, wu:fc14d07 Kit ELISA, zgc:65860 Kit ELISA, zgc:77389 Kit ELISA, SRY-box2 Kit ELISA, Xsox-2 Kit ELISA, anop3 Kit ELISA, mcops3 Kit ELISA, sox-2 Kit ELISA, SRY-box 2 Kit ELISA, SRY box 2 Kit ELISA, SRY (sex determining region Y)-box 2 Kit ELISA, SRY-box 2 S homeolog Kit ELISA, SOX2 Kit ELISA, Sox2 Kit ELISA, sox2 Kit ELISA, sox2.S Kit ELISA
    ID gène
    6657
    UniProt
    P48431
    Pathways
    Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis
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