CSTB Kit ELISA

N° du produit ABIN5564570

Détail du produit CSTB Kit ELISA

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.0938-6 ng/mL

Seuil minimal de détection: 0.0938 ng/mL

Application: ELISA

Fonction: The AssayMax™ Human Cystatin-B ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of cystatin-B in human plasma, serum, urine, saliva, milk, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human cystatin-B in approximately 4 hours. A polyclonal antibody specific for human cystatin-B has been pre-coated onto a 96-well microplate with removable strips. Cystatin-B in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human cystatin-B, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Marque: AssayMax™

Type d'échantillon: Cell Culture Cells, Cerebrospinal Fluid, Milk, Plasma, Saliva, Serum, Urine

Analytical Method: Quantitative

Ingrédients: Human Cystatin-B Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human cystatin-B. Sealing Tapes: Each kit contains 3 precut, pressure sensitive sealing tapes that can be cut to fit the format of the individual assay. Human Cystatin-B Standard: Human cystatin-B in a buffered protein base (6 ng, lyophilized, 2 vials). Biotinylated Human Cystatin-B Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against human cystatin-B (120 l). MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base (30 ml). Standard Diluent (1x): A buffered protein base with stabilizer (2 ml). Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant (30 ml, 2 bottles). SP Conjugate (100x): A 100-fold concentrate (80 l). Chromogen Substrate (1x): A stabilized peroxidase chromogen substrate tetramethylbenzidine (8 ml). Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substrate reaction (12 ml).

Matériel non inclus: Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)

Information d'application

Plaque: Pre-coated

Protocole:

• Step 1. Add 50 μL of Standard or Sample per well. Incubate 2 hours.
• Step 2. Wash, then add 50 μL of Biotinylated Antibody per well. Incubate 1 hour.
• Step 3. Wash, then add 50 μL of SP Conjugate per well. Incubate 30 minutes.
• Step 4. Wash, then add 50 μL of Chromogen Substrate per well. Incubate 30 minutes.
• Step 5. Add 50 μL of Stop Solution per well. Read at 450 nm immediately.

Préparation des réactifs:

Freshly dilute all reagents and bring all reagents to room temperature before use. MIX Diluent Concentrate (10x): If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved. Dilute the MIX Diluent Concentrate 10-fold with reagent grade water to produce a 1x solution. Store for up to 30 days at 2-8 °C. Human Cystatin-B Standard: Reconstitute the Human Cystatin-B Standard (6 ng) with 0.5 mL of Standard Diluent to generate a 12 ng/mL standard stock solution. Allow the vial to sit for 10 minutes with gentle agitation prior to making dilutions. Prepare duplicate or triplicate standard points by serially diluting from the standard stock solution (12 ng/mL) 2-fold with equal volume of MIX Diluent to produce 6, 3, 1.5, 0.75, 0.375, 0.188, and 0.094 ng/mL solutions. MIX Diluent serves as the zero standard (0 ng/mL). Aliquot remaining stock solution to limit repeated freeze-thaw cycles. This solution should be stored at -20 °C and used within 48 hours. 5 Standard Point Dilution [Cystatin-B] (ng/mL) P1 1 part Standard (12 ng/mL) + 1 part MIX Diluent 6.0 P2 1 part P1 + 1 part MIX Diluent 3.0 P3 1 part P2 + 1 part MIX Diluent 1.5 P4 1 part P3 + 1 part MIX Diluent 0.75 P5 1 part P4 + 1 part MIX Diluent 0.375 P6 1 part P5 + 1 part MIX Diluent 0.188 P7 1 part P6 + 1 part MIX Diluent 0.094 P8 MIX Diluent 0.0 Biotinylated Human Cystatin-B Antibody (50x): Spin down the antibody briefly and dilute the desired amount of the antibody 50-fold with MIX Diluent to produce a 1x solution. The undiluted antibody should be stored at -20 °C. Wash Buffer Concentrate (20x): If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved. Dilute the Wash Buffer Concentrate 20-fold with reagent grade water to produce a 1x solution. SP Conjugate (100x): Spin down the SP Conjugate briefly and dilute the desired amount of the conjugate 100-fold with MIX Diluent to produce a 1x solution. The undiluted conjugate should be stored at -20 °C.

Prélèvement de l'échantillon: Plasma: Collect plasma using one-tenth volume of 0.1 M sodium citrate as an anticoagulant. Centrifuge samples at 3000 x g for 10 minutes and collect plasma. A 6-fold sample dilution is suggested into MIX Diluent, however, user should determine optimal dilution factor depending on application needs. The undiluted samples can be stored at -20 °C or below for up to 3 months. Avoid repeated freeze-thaw cycles (EDTA or Heparin can also be used as an anticoagulant). Serum: Samples should be collected into a serum separator tube. After clot formation, centrifuge samples at 3000 x g for 10 minutes and remove serum. A 6-fold sample dilution is suggested into MIX Diluent, however, user should determine optimal dilution factor depending on application needs. The undiluted samples can be stored at -20 °C or below for up to 3 months. Avoid repeated freeze-thaw cycles. Urine: Collect urine using sample pot. Centrifuge samples at 800 x g for 10 minutes. A 2-fold sample dilution is suggested into MIX Diluent, however, user should determine optimal dilution factor depending on application needs. The undiluted samples can be stored at -20 °C or below for up to 3 months. Avoid repeated freeze-thaw cycles. Saliva: Collect saliva using sample tube. Centrifuge samples at 800 x g for 10 minutes. A 200-fold sample dilution is suggested into MIX Diluent or within the range of 50x - 500x, however, user should determine optimal dilution factor depending on application needs. The undiluted samples can be stored at -20 °C or below for up to 3 months. Avoid repeated freeze-thaw cycles. Milk: Collect milk using sample tube. Centrifuge samples at 800 x g for 10 minutes. A 100-fold sample dilution is suggested into MIX Diluent, however, user should determine optimal dilution factor depending on application needs. The undiluted samples can be stored at -20 °C or below for up to 3 months. Avoid repeated freeze-thaw cycles. CSF: Collect cerebrospinal fluid (CSF) using sample pot. Centrifuge samples at 3000 x g for 10 minutes. A 10-fold sample dilution is suggested into MIX Diluent, however, user should determine optimal dilution factor depending on application needs. The undiluted samples can be stored at -80 °C for up to 3 months. Avoid repeated freeze-thaw cycles. 4 Cell Culture Supernatants: Centrifuge cell culture media at 3000 x g for 10 minutes at 4 °C to remove debris and collect supernatants. Samples can be stored at -20 °C or below. Avoid repeated freeze-thaw cycles.

Procédure de l'essai:

Prepare all reagents, standard solutions, and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature (20-25 °C). Remove excess microplate strips from the plate frame and return them immediately to the foil pouch with desiccants inside. Reseal the pouch securely to minimize exposure to water vapor and store in a vacuum desiccator. Add 50 l of Human Cystatin-B Standard or sample to each well. Gently tap plate to thoroughly coat the wells. Break any bubbles that may have formed. Cover wells with a sealing tape and incubate for 2 hours. Start the timer after the last addition. Wash five times with 200 l of Wash Buffer manually. Invert the plate each time and decant the contents, hit 4-5 times on absorbent material to completely remove the liquid. If using a machine, wash six times with 300 l of Wash Buffer and then invert the plate, decanting the contents, hit 4-5 times on absorbent material to completely remove the liquid. Add 50 l of Biotinylated Human Cystatin-B Antibody to each well. Gently tap plate to thoroughly coat the wells. Break any bubbles that 6 may have formed. Cover wells with a sealing tape and incubate for 1 hour. Wash the microplate as described above. Add 50 l of SP Conjugate to each well. Gently tap plate to thoroughly coat the wells. Break any bubbles that may have formed. Cover wells with a sealing tape and incubate for 30 minutes. Turn on the microplate reader and set up the program in advance. Wash the microplate as described above. Add 50 l of Chromogen Substrate to each well. Gently tap plate to thoroughly coat the wells. Break any bubbles that may have formed. Incubate for 30 minutes or until the optimal blue color density develops. Add 50 l of Stop Solution to each well. The color will change from blue to yellow. Gently tap plate to ensure thorough mixing. Break any bubbles that may have formed. Read the absorbance on a microplate reader at a wavelength of 450 nm immediately. If wavelength correction is available, subtract readings at 570 nm from those at 450 nm to correct optical imperfections. Otherwise, read the plate at 450 nm only. Please note that some unstable black particles may be generated at high concentration points after stopping the reaction for about 10 minutes, which will reduce the readings.

Calcul des résultats:

• Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
• To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
• Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: This product is for Research Use Only and is not intended for use in diagnostic procedures. Prepare all reagents (diluent buffer, wash buffer, standard, biotinylated antibody, and SP conjugate) as instructed, prior to running the assay. 2 Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date.

Stock: 4 °C,-20 °C

Stockage commentaire: Upon arrival, immediately store components of the kit at recommended temperatures up to the expiration date. Store Standard, SP Conjugate, and Biotinylated Antibody at -20°C. Store Microplate, Diluent Concentrate (10x), Standard Diluent (1x), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8°C. Unused microplate wells may be returned to the foil pouch with the desiccant packs and resealed. May be stored for up to 30 days in a vacuum desiccator. 3

Détail du antigène

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Autre désignation: Cystatin-B (CSTB) (CSTB Produits)

Synonymes: CST6 Kit ELISA, EPM1 Kit ELISA, EPM1A Kit ELISA, PME Kit ELISA, STFB Kit ELISA, ULD Kit ELISA, CSTB Kit ELISA, Cyb Kit ELISA, Epm1 Kit ELISA, Stfb Kit ELISA, MGC189005 Kit ELISA, pme Kit ELISA, cst6 Kit ELISA, epm1 Kit ELISA, stfb Kit ELISA, AA960480 Kit ELISA, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Kit ELISA, ATCYS6 Kit ELISA, ATCYSB Kit ELISA, cystatin B Kit ELISA, LOC100008600 Kit ELISA, LOC100136235 Kit ELISA, CPI1 Kit ELISA, cystatin B Kit ELISA, cystatin B (stefin B) Kit ELISA, cystatin 14a, tandem duplicate 2 Kit ELISA, cystatin-B Kit ELISA, CSTB Kit ELISA, Cstb Kit ELISA, cstb Kit ELISA, cst14a.2 Kit ELISA, CYSB Kit ELISA, cpi1 Kit ELISA, LOC101123265 Kit ELISA

Sujet: Cystatin-B (CSTB), also called CPI-B, stefin-B, and liver thiol proteinase inhibitor, is a small protein that is a member of the superfamily of cysteine protease inhibitors. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Cystatins have been classified into three types: type 1 stefins, type 2 cystatins, and type 3 kininogens. Cystatin-B belongs to the type 1 stefins which are mainly intracellular proteins, whereas the cystatins and the kininogens are extracellular. The 98-aa and 11 kDa cystatin-B protein is localized in the cytosol, mitochondria, and nucleus in order to function as a protector against the proteinases leaking from lysosomes. It is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsin (1-2). Cystatin-B is upregulated upon macrophage activation and cellular stress. It plays a role in neuro-inflammation (3).

ID gène: 1476

UniProt: P04080

Pathways: Response to Water Deprivation