Transglutaminase 2 Kit ELISA

N° du produit ABIN5564717

Détail du produit Transglutaminase 2 Kit ELISA

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of High Sensitive Transglutaminase 2, Tissue (TGM2).
No significant cross-reactivity or interference between High Sensitive Transglutaminase 2, Tissue (TGM2) and analogues was observed.

Sensibilité: 0.063 ng/mL

Classe de qualité: High Sensitivity

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard Diluent
• Assay Diluent A
• Assay Diluent B
• Stop Solution
• Standard
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Wash Buffer (30 x concentrate)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Transglutaminase 2, Tissue (TGM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Transglutaminase 2, Tissue (TGM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Transglutaminase 2, Tissue (TGM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Transglutaminase 2, Tissue (TGM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Procédure de l'essai:

1. Prepare all reagents, samples and standards,
   
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
   
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
   
4. Aspirate and wash 3 times,
   
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
   
6. Aspirate and wash 5 times,
   
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
   
8. Add 50μL Stop Solution. Read at 450nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Transglutaminase 2, Tissue (TGM2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Transglutaminase 2, Tissue (TGM2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: Transglutaminase 2, Tissue (TGM2) (TGM2 Produits)

Synonymes: G-ALPHA-h Kit ELISA, GNAH Kit ELISA, TG2 Kit ELISA, TGC Kit ELISA, tgm2 Kit ELISA, zgc:101081 Kit ELISA, TgaseII Kit ELISA, TGM2 Kit ELISA, fc12b04 Kit ELISA, wu:fc12b04 Kit ELISA, zgc:136700 Kit ELISA, TGL2 Kit ELISA, G[a]h Kit ELISA, TGase2 Kit ELISA, tTG Kit ELISA, tTGas Kit ELISA, transglutaminase 2 Kit ELISA, transglutaminase 2, C polypeptide A Kit ELISA, transglutaminase 2b Kit ELISA, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) Kit ELISA, transglutaminase 2, C polypeptide Kit ELISA, TGM2 Kit ELISA, tgm2a Kit ELISA, Tgm2 Kit ELISA, tgm2b Kit ELISA

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling