Exosome Purification and Analysis Kit (Cell culture)
-
- Reactivité
- Humain
- Méthode de détection
- Fluorometric
- Conjugué
- PE
- Application
- Flow Cytometry (FACS)
- Fonction
- Exosome purification and FACS analysis kit of exosomes derived from cell culture
- Marque
- ExoStep™
- Type d'échantillon
- Cell Culture Cells
- Attributs du produit
- The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
- Ingrédients
- CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
- Matériel non inclus
-
• Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
- tubes
). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
-
Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivité: Souris Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA KitReactivité: Différentes espèces Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 6 (IL6) ELISA KitIL6 Reactivité: Humain Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivité: Humain Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivité: Humain AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivité: Humain Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- Indications d'application
- Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes
- Commentaires
-
Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol
- Volume d'échantillon
- 50 μL
- Durée du test
- 4 h
- Préparation des réactifs
-
Reagents ready to use.
- Restrictions
- For Research Use only
-
- Buffer
- The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store in the dark at 2-8 °C
-
- Sujet
- Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
-
