DHCR7 Kit ELISA

N° du produit ABIN5651296

Détail du produit DHCR7 Kit ELISA

Antigène: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of 7-Dehydrocholesterol Reductase (DHCR7). No significant cross-reactivity or interference between 7-Dehydrocholesterol Reductase (DHCR7) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 7-Dehydrocholesterol Reductase (DHCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 7-Dehydrocholesterol Reductase (DHCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Autre désignation: 7-Dehydrocholesterol Reductase (DHCR7 Produits)

Synonymes: 7-Dhcr Kit ELISA, Xdhcr7 Kit ELISA, MGC64361 Kit ELISA, slos Kit ELISA, 7-STEROL DELTA7 REDUCTASE Kit ELISA, 7RED Kit ELISA, DELTA5 Kit ELISA, DWARF 5 Kit ELISA, F11F12.21 Kit ELISA, F11F12_21 Kit ELISA, LE Kit ELISA, LEPIDA Kit ELISA, PA Kit ELISA, PARVA Kit ELISA, ST7R Kit ELISA, STEROL DELTA7 REDUCTASE Kit ELISA, DKFZp468C1911 Kit ELISA, AA409147 Kit ELISA, SLOS Kit ELISA, xdhcr7 Kit ELISA, 7-dehydrocholesterol reductase Kit ELISA, 7-dehydrocholesterol reductase L homeolog Kit ELISA, Ergosterol biosynthesis ERG4/ERG24 family Kit ELISA, DHCR7 Kit ELISA, dhcr7 Kit ELISA, dhcr7.L Kit ELISA, DWF5 Kit ELISA, PAAG_04766 Kit ELISA, PITG_13128 Kit ELISA, VDBG_05423 Kit ELISA, MGYG_00712 Kit ELISA, TERG_00580 Kit ELISA, Dhcr7 Kit ELISA

Sujet:

Gene Name: 7-Dehydrocholesterol Reductase

Gene Aliases: DHCR-7, SLOS, D7SR, Smith-Lemli-Opitz Syndrome, Putative sterol reductase SR-2, Sterol Delta(7)-reductase

ID gène: 1717

UniProt: Q9UBM7