ABI1 Kit ELISA

N° du produit ABIN5651333

Détail du produit ABI1 Kit ELISA

Antigène: ABI1 (Abl-Interactor 1 (ABI1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Abl Interactor 1 (ABI1). No significant cross-reactivity or interference between Abl Interactor 1 (ABI1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Abl Interactor 1 (ABI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Abl Interactor 1 (ABI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Abl Interactor 1 (ABI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Abl Interactor 1 (ABI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Abl Interactor 1 (ABI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ABI1 (Abl-Interactor 1 (ABI1))

Autre désignation: Abl Interactor 1 (ABI1 Produits)

Synonymes: ABI-1 Kit ELISA, ABLBP4 Kit ELISA, E3B1 Kit ELISA, NAP1BP Kit ELISA, SSH3BP Kit ELISA, SSH3BP1 Kit ELISA, NAP1 Kit ELISA, Ssh3bp1 Kit ELISA, E3b1 Kit ELISA, abi1 Kit ELISA, wu:fc66g09 Kit ELISA, wu:fk56a10 Kit ELISA, zgc:73174 Kit ELISA, MGC84356 Kit ELISA, ABI1 Kit ELISA, abi-1 Kit ELISA, e3b1 Kit ELISA, nap1bp Kit ELISA, ssh3bp Kit ELISA, ssh3bp1 Kit ELISA, wu:fe48a10 Kit ELISA, zgc:153534 Kit ELISA, DKFZp469P1319 Kit ELISA, abl interactor 1 Kit ELISA, abl-interactor 1 Kit ELISA, abl-interactor 1a Kit ELISA, abl interactor 1 S homeolog Kit ELISA, abl-interactor 1b Kit ELISA, Abl-interactor 1 Kit ELISA, Abl interactor 1 Kit ELISA, ABI1 Kit ELISA, Abi1 Kit ELISA, abi1a Kit ELISA, abi1.S Kit ELISA, abi1 Kit ELISA, abi1b Kit ELISA, Bm1_44660 Kit ELISA, LOAG_04356 Kit ELISA

Sujet:

Gene Name: Abl Interactor 1

Gene Aliases: ABI-1, E3B1, NAP1BP, SSH3BP1, Spectrin SH3 Domain Binding Protein 1, Abelson interactor 1, Abl-binding protein 4, Nap1-binding protein, Eps8 SH3 domain-binding protein

ID gène: 10006

UniProt: Q8IZP0

Pathways: Signalisation RTK, Response to Water Deprivation, ER-Nucleus Signaling