ATF4 Kit ELISA
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- Antigène Voir toutes ATF4 Kits ELISA
- ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.12 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78.12 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Activating Transcription Factor 4 (ATF4). No significant cross-reactivity or interference between Activating Transcription Factor 4 (ATF4) and analogues was observed.
- Sensibilité
- 28 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Transcription Factor 4 (ATF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Transcription Factor 4 (ATF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Transcription Factor 4 (ATF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Transcription Factor 4 (ATF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activating Transcription Factor 4 (ATF4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activating Transcription Factor 4 (ATF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes ATF4 Kits ELISA
- ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
- Autre désignation
- Activating Transcription Factor 4 (ATF4 Produits)
- Synonymes
- CREB-2 Kit ELISA, CREB2 Kit ELISA, TAXREB67 Kit ELISA, TXREB Kit ELISA, ATF4 Kit ELISA, atf-4 Kit ELISA, creb-2 Kit ELISA, creb2 Kit ELISA, taxreb67 Kit ELISA, txreb Kit ELISA, atf4 Kit ELISA, wu:fj01c08 Kit ELISA, wu:fk30b04 Kit ELISA, zgc:85951 Kit ELISA, sb:eu681 Kit ELISA, wu:fb08f07 Kit ELISA, zgc:171702 Kit ELISA, Atf-4 Kit ELISA, C/ATF Kit ELISA, ATF4-I Kit ELISA, atf4-a Kit ELISA, atf4-b Kit ELISA, atf4-ii Kit ELISA, activating transcription factor 4 Kit ELISA, activating transcription factor 4 L homeolog Kit ELISA, activating transcription factor 4a Kit ELISA, activating transcription factor 4b Kit ELISA, activating transcription factor 4 S homeolog Kit ELISA, ATF4 Kit ELISA, atf4 Kit ELISA, Atf4 Kit ELISA, atf4.L Kit ELISA, atf4a Kit ELISA, atf4b Kit ELISA, atf4.S Kit ELISA
- Sujet
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Gene Name: Activating Transcription Factor 4
Gene Aliases: TAXREB67, TXREB, CREB-2, DNA-binding protein TAXREB67, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67
- ID gène
- 79255
- UniProt
- Q9ES19
- Pathways
- Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
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