AP2M1 Kit ELISA

N° du produit ABIN5651410

Détail du produit AP2M1 Kit ELISA

Antigène: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adaptor Related Protein Complex 2 Mu 1 (AP2m1). No significant cross-reactivity or interference between Adaptor Related Protein Complex 2 Mu 1 (AP2m1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 2 Mu 1 (AP2m1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 2 Mu 1 (AP2m1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adaptor Related Protein Complex 2 Mu 1 (AP2m1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adaptor Related Protein Complex 2 Mu 1 (AP2m1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))

Autre désignation: Adaptor Related Protein Complex 2 Mu 1 (AP2M1 Produits)

Synonymes: AP50 Kit ELISA, CLAPM1 Kit ELISA, mu2 Kit ELISA, Ap50 Kit ELISA, Clapm1 Kit ELISA, cb34 Kit ELISA, Ap2m1 Kit ELISA, dpy-23 Kit ELISA, sb:cb34 Kit ELISA, zgc:55711 Kit ELISA, zgc:85653 Kit ELISA, wu:fa97a10 Kit ELISA, ap2m1-a Kit ELISA, ap2m1 Kit ELISA, zgc:56643 Kit ELISA, AP2M1 Kit ELISA, adaptor related protein complex 2 mu 1 subunit Kit ELISA, adaptor-related protein complex 2, mu 1 subunit Kit ELISA, adaptor-related protein complex 2, mu 1 subunit, a Kit ELISA, adaptor related protein complex 2 mu 1 subunit L homeolog Kit ELISA, adaptor-related protein complex 2, mu 1 subunit, b Kit ELISA, AP-2 complex subunit mu-1 Kit ELISA, clathrin coat assembly protein AP50 Kit ELISA, clathrin coat assembly protein ap50 Kit ELISA, AP2M1 Kit ELISA, Ap2m1 Kit ELISA, ap2m1a Kit ELISA, ap2m1.L Kit ELISA, ap2m1b Kit ELISA, ap2m1 Kit ELISA, cgd8_950 Kit ELISA, PY06523 Kit ELISA, PVX_118455 Kit ELISA, PVX_123590 Kit ELISA, CpipJ_CPIJ003697 Kit ELISA

Sujet:

Gene Name: Adaptor Related Protein Complex 2 Mu 1

Gene Aliases: AP50, CLAPM1, Clathrin-Associated/Assembly/Adaptor Protein,Medium 1, Plasma Membrane Adaptor AP-2 50kDA Protein, Clathrin Coat Adaptor Protein AP50

ID gène: 1173

UniProt: Q96CW1

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome