APC Kit ELISA

N° du produit ABIN5651414

Détail du produit APC Kit ELISA

Antigène: APC (Adenomatous Polyposis Coli (APC))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adenomatosis Polyposis Coli Protein (APC). No significant cross-reactivity or interference between Adenomatosis Polyposis Coli Protein (APC) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenomatosis Polyposis Coli Protein (APC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenomatosis Polyposis Coli Protein (APC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenomatosis Polyposis Coli Protein (APC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenomatosis Polyposis Coli Protein (APC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenomatosis Polyposis Coli Protein (APC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenomatosis Polyposis Coli Protein (APC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: APC (Adenomatous Polyposis Coli (APC))

Autre désignation: Adenomatosis Polyposis Coli Protein (APC Produits)

Synonymes: dp2 Kit ELISA, dp3 Kit ELISA, fap Kit ELISA, fpc Kit ELISA, xapc Kit ELISA, dp2.5 Kit ELISA, AI047805 Kit ELISA, AU020952 Kit ELISA, AW124434 Kit ELISA, CC1 Kit ELISA, Min Kit ELISA, mAPC Kit ELISA, BTPS2 Kit ELISA, DP2 Kit ELISA, DP2.5 Kit ELISA, DP3 Kit ELISA, GS Kit ELISA, PPP1R46 Kit ELISA, RATAPC Kit ELISA, APC1 Kit ELISA, APC, WNT signaling pathway regulator Kit ELISA, adenomatous polyposis coli Kit ELISA, adenomatous polyposis coli protein, putative Kit ELISA, adenomatosis polyposis coli Kit ELISA, adenomatous polyposis coli L homeolog Kit ELISA, APC Kit ELISA, apc Kit ELISA, Smp_139190 Kit ELISA, Apc Kit ELISA, apc.L Kit ELISA

Sujet:

Gene Name: Adenomatosis Polyposis Coli Protein

Gene Aliases: DP2, DP2.5, DP3, FAP, FPC, GS, Deleted in polyposis 2.5

UniProt: Q61315

Pathways: Signalisation WNT, Stem Cell Maintenance, Cell-Cell Junction Organization