AHCY Kit ELISA

N° du produit ABIN5651426

Détail du produit AHCY Kit ELISA

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adenosylhomocysteinase (AHCY). No significant cross-reactivity or interference between Adenosylhomocysteinase (AHCY) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosylhomocysteinase (AHCY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosylhomocysteinase (AHCY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosylhomocysteinase (AHCY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosylhomocysteinase (AHCY) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenosylhomocysteinase (AHCY) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenosylhomocysteinase (AHCY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Autre désignation: Adenosylhomocysteinase (AHCY Produits)

Synonymes: sahh Kit ELISA, PSPTO5068 Kit ELISA, Ahcy Kit ELISA, SAHH Kit ELISA, adoHcyase Kit ELISA, ahcy Kit ELISA, AA987153 Kit ELISA, AL024110 Kit ELISA, CuBP Kit ELISA, D150 Kit ELISA, cb1079 Kit ELISA, hm:zeh1173 Kit ELISA, hm:zeh1364 Kit ELISA, wu:fj67b02 Kit ELISA, adenosylhomocysteinase Kit ELISA, Adenosylhomocysteinase Kit ELISA, S-adenosylhomocysteine hydrolase Kit ELISA, adenosylhomocysteinase S homeolog Kit ELISA, AHCY Kit ELISA, ahcy Kit ELISA, ahcY Kit ELISA, MMAH_RS02605 Kit ELISA, Srot_2217 Kit ELISA, Ndas_3755 Kit ELISA, Deba_0936 Kit ELISA, Palpr_2392 Kit ELISA, Calni_0569 Kit ELISA, LOC100282150 Kit ELISA, Intca_2510 Kit ELISA, Marky_0392 Kit ELISA, Desac_1394 Kit ELISA, Tc00.1047053511229.50 Kit ELISA, Tc00.1047053511589.200 Kit ELISA, Tb11.01.1350 Kit ELISA, LMJF_36_3910 Kit ELISA, MCYG_06244 Kit ELISA, Ahcy Kit ELISA, ahcy.S Kit ELISA

Sujet:

Gene Name: Adenosylhomocysteinase

Gene Aliases: SAHH, AdoHcyase, S-Adenosylhomocysteine Hydrolase, S-adenosyl-L-homocysteine hydrolase

ID gène: 191

UniProt: P23526