Adenylate Kinase 3 Kit ELISA

N° du produit ABIN5651437

Détail du produit Adenylate Kinase 3 Kit ELISA

Antigène: Adenylate Kinase 3 (AK3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cerebrospinal Fluid, Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adenylate Kinase 3 (AK3). No significant cross-reactivity or interference between Adenylate Kinase 3 (AK3) and analogues was observed.

Sensibilité: 0.146 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Kinase 3 (AK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Kinase 3 (AK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Kinase 3 (AK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Kinase 3 (AK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Kinase 3 (AK3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Kinase 3 (AK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Adenylate Kinase 3 (AK3)

Autre désignation: Adenylate Kinase 3 (AK3 Produits)

Synonymes: AK3L1 Kit ELISA, AK6 Kit ELISA, AKL3L Kit ELISA, AKL3L1 Kit ELISA, FIX Kit ELISA, AK4 Kit ELISA, AA407498 Kit ELISA, AI506714 Kit ELISA, AK-3 Kit ELISA, Ak3l Kit ELISA, Ak3l1 Kit ELISA, Akl3l Kit ELISA, ak3l1 Kit ELISA, sb:cb845 Kit ELISA, zgc:64135 Kit ELISA, id:ibd3034 Kit ELISA, wu:fa02c11 Kit ELISA, wu:fc20h08 Kit ELISA, AK3 Kit ELISA, CG6612 Kit ELISA, DAK3 Kit ELISA, Dak3 Kit ELISA, Dmel\\CG6612 Kit ELISA, bs12h03.y1 Kit ELISA, MGC114646 Kit ELISA, ak6 Kit ELISA, adenylate kinase 3 Kit ELISA, Adenylate kinase 3 Kit ELISA, adenylate kinase 3 L homeolog Kit ELISA, AK3 Kit ELISA, Ak3 Kit ELISA, ak3 Kit ELISA, Adk3 Kit ELISA, ak3.L Kit ELISA, ADK3 Kit ELISA

Sujet:

Gene Name: Adenylate Kinase 3

Gene Aliases: AK3L1, AK6, AKL3L, Adenylate Kinase 3 Alpha-Like 1, GTP:AMP Phosphotransferase AK3, Mitochondrial

ID gène: 50808

UniProt: Q9UIJ7

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process