AF4 Kit ELISA

N° du produit ABIN5651515

Détail du produit AF4 Kit ELISA

Antigène: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of AF4/FMR2 Family, Member 1 (AFF1). No significant cross-reactivity or interference between AF4/FMR2 Family, Member 1 (AFF1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AF4/FMR2 Family, Member 1 (AFF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AF4/FMR2 Family, Member 1 (AFF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AF4/FMR2 Family, Member 1 (AFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AF4/FMR2 Family, Member 1 (AFF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AF4/FMR2 Family, Member 1 (AFF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))

Autre désignation: AF4/FMR2 Family, Member 1 (AFF1 Produits)

Synonymes: AF4 Kit ELISA, MLLT2 Kit ELISA, PBM1 Kit ELISA, 9630032B01Rik Kit ELISA, AW319193 Kit ELISA, Af4 Kit ELISA, Mllt2h Kit ELISA, Rob Kit ELISA, Mllt2 Kit ELISA, AF4/FMR2 family member 1 Kit ELISA, AF4/FMR2 family, member 1 Kit ELISA, AFF1 Kit ELISA, Aff1 Kit ELISA

Sujet:

Gene Name: AF4/FMR2 Family, Member 1

Gene Aliases: AF-4, AF4, AF4-MLL, MLL/AF4, MLLT2, PBM1, Myeloid/Lymphoid Or Mixed-Lineage Leukemia, Translocated To 2, Pre-B-Cell Monocytic Leukemia Partner 1

ID gène: 4299

UniProt: P51825