ALDH9A1 Kit ELISA

N° du produit ABIN5651561

Détail du produit ALDH9A1 Kit ELISA

Antigène: ALDH9A1 (Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1). No significant cross-reactivity or interference between Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) and analogues was observed.

Sensibilité: 0.066 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ALDH9A1 (Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1))

Autre désignation: Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1 Produits)

Synonymes: ALDH4 Kit ELISA, ALDH7 Kit ELISA, ALDH9 Kit ELISA, E3 Kit ELISA, TMABADH Kit ELISA, Ahd-2 Kit ELISA, Ahd2 Kit ELISA, Aldh1 Kit ELISA, Aldh1a2 Kit ELISA, E1 Kit ELISA, Raldh1 Kit ELISA, Tmaba-dh Kit ELISA, MGC52697 Kit ELISA, aldh9a1 Kit ELISA, ALDH9A1 Kit ELISA, ECK1438 Kit ELISA, JW1439 Kit ELISA, prr Kit ELISA, ydcW Kit ELISA, SC6A9.01c Kit ELISA, SC8B7.12c Kit ELISA, AA139417 Kit ELISA, Abaldh Kit ELISA, ESTM40 Kit ELISA, TMABA-DH Kit ELISA, Tmabadh Kit ELISA, aldh9 Kit ELISA, aldehyde dehydrogenase 9 family member A1 Kit ELISA, aldehyde dehydrogenase family 1, subfamily A1 Kit ELISA, aldehyde dehydrogenase 9 family, member A1 Kit ELISA, aldehyde dehydrogenase 9 family member A1 S homeolog Kit ELISA, aldehyde dehydrogenase 9 family, member A1b Kit ELISA, gamma-aminobutyraldehyde dehydrogenase Kit ELISA, aldehyde dehydrogenase Kit ELISA, aldehyde dehydrogenase 9, subfamily A1 Kit ELISA, betaine aldehyde dehydrogenase Kit ELISA, ALDH9A1 Kit ELISA, Aldh1a1 Kit ELISA, Aldh9a1 Kit ELISA, aldh9a1.S Kit ELISA, aldh9a1b Kit ELISA, aldh9a1 Kit ELISA, ECs2048 Kit ELISA, patD Kit ELISA, Z2275 Kit ELISA, SCO5666 Kit ELISA, SAV_2592 Kit ELISA, SMc01656 Kit ELISA, STY1467 Kit ELISA, ECA1542 Kit ELISA, CYA_0364 Kit ELISA, CYB_0715 Kit ELISA, SGR_RS09015 Kit ELISA, ydcW Kit ELISA, APA01_RS11285 Kit ELISA, CTU_20580 Kit ELISA, Sros_1880 Kit ELISA

Sujet:

Gene Name: Aldehyde Dehydrogenase 9 Family, Member A1

Gene Aliases: ALDH4, ALDH7, ALDH9, E3, TMABADH, 4-Trimethylaminobutyraldehyde Dehydrogenase, Aldehyde dehydrogenase E3 isozyme, Gamma-aminobutyraldehyde dehydrogenase

ID gène: 56752

UniProt: Q9JLJ2