AMACR Kit ELISA

N° du produit ABIN5651657

Détail du produit AMACR Kit ELISA

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Alpha-Methylacyl Coenzyme A Racemase (aMACR). No significant cross-reactivity or interference between Alpha-Methylacyl Coenzyme A Racemase (aMACR) and analogues was observed.

Sensibilité: 0.066 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Methylacyl Coenzyme A Racemase (aMACR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Methylacyl Coenzyme A Racemase (aMACR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Methylacyl Coenzyme A Racemase (aMACR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Methylacyl Coenzyme A Racemase (aMACR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Methylacyl Coenzyme A Racemase (aMACR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Autre désignation: Alpha-Methylacyl Coenzyme A Racemase (AMACR Produits)

Synonymes: AMACR Kit ELISA, DKFZp469O1232 Kit ELISA, amacr Kit ELISA, NCU04099.1 Kit ELISA, MGC89832 Kit ELISA, AMACRD Kit ELISA, CBAS4 Kit ELISA, RACE Kit ELISA, RM Kit ELISA, Macr1 Kit ELISA, Da1-8 Kit ELISA, Marc1 Kit ELISA, alpha-methylacyl-CoA racemase Kit ELISA, PROBABLE ALPHA-METHYLACYL-COA RACEMASE MCR (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase) Kit ELISA, Alpha-methylacyl-CoA racemase Kit ELISA, alpha-methylacyl-CoA racemase L homeolog Kit ELISA, AMACR Kit ELISA, mcr Kit ELISA, BPSL0064 Kit ELISA, Maqu_2037 Kit ELISA, UREG_03852 Kit ELISA, MCYG_01809 Kit ELISA, amacr Kit ELISA, amacr.L Kit ELISA, MGYG_04259 Kit ELISA, NCU04099 Kit ELISA, Amacr Kit ELISA

Sujet:

Gene Name: Alpha-Methylacyl Coenzyme A Racemase

Gene Aliases: RACE, 2-methylacyl-CoA racemase

ID gène: 23600

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process