Anillin Kit ELISA

N° du produit ABIN5651818

Détail du produit Anillin Kit ELISA

Antigène: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Anillin (ANLN). No significant cross-reactivity or interference between Anillin (ANLN) and analogues was observed.

Sensibilité: 11.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anillin (ANLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anillin (ANLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anillin (ANLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anillin (ANLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anillin (ANLN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anillin (ANLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Autre désignation: Anillin (ANLN Produits)

Synonymes: Scraps Kit ELISA, scra Kit ELISA, ANLN Kit ELISA, 1110037A17Rik Kit ELISA, 2900037I21Rik Kit ELISA, C78101 Kit ELISA, scraps Kit ELISA, ABP8 Kit ELISA, Abp8 Kit ELISA, Ani Kit ELISA, Anil Kit ELISA, Anillin Kit ELISA, CG2092 Kit ELISA, Dmel\\CG2092 Kit ELISA, Scra Kit ELISA, ani Kit ELISA, anil Kit ELISA, anillin Kit ELISA, l(2)03427 Kit ELISA, l(2)43Ec Kit ELISA, l(2)k08255 Kit ELISA, RGD1561367 Kit ELISA, si:ch211-197n10.2 Kit ELISA, wu:fb74d05 Kit ELISA, wu:fd10d09 Kit ELISA, wu:fi41f04 Kit ELISA, anillin actin binding protein Kit ELISA, anillin, actin binding protein Kit ELISA, scraps Kit ELISA, anillin actin binding protein L homeolog Kit ELISA, ANLN Kit ELISA, Anln Kit ELISA, anln Kit ELISA, scra Kit ELISA, anln.L Kit ELISA

Sujet:

Gene Name: Anillin

Gene Aliases: Scra, Scraps, Actin-Binding Protein Anillin