APEX1 Kit ELISA

N° du produit ABIN5651973

Détail du produit APEX1 Kit ELISA

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Apurinic/Apyrimidinic Endonuclease 1 (APEX1). No significant cross-reactivity or interference between Apurinic/Apyrimidinic Endonuclease 1 (APEX1) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apurinic/Apyrimidinic Endonuclease 1 (APEX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apurinic/Apyrimidinic Endonuclease 1 (APEX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apurinic/Apyrimidinic Endonuclease 1 (APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apurinic/Apyrimidinic Endonuclease 1 (APEX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apurinic/Apyrimidinic Endonuclease 1 (APEX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apurinic/Apyrimidinic Endonuclease 1 (APEX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: Apurinic/Apyrimidinic Endonuclease 1 (APEX1 Produits)

Synonymes: apex1 Kit ELISA, MGC76280 Kit ELISA, MGC79645 Kit ELISA, MGC78928 Kit ELISA, APE Kit ELISA, APE1 Kit ELISA, APEN Kit ELISA, APEX Kit ELISA, APX Kit ELISA, HAP1 Kit ELISA, REF1 Kit ELISA, Apex Kit ELISA, REF-1 Kit ELISA, BAP1 Kit ELISA, apex1a Kit ELISA, hm:zehn1532 Kit ELISA, wu:fc13d11 Kit ELISA, zgc:66204 Kit ELISA, Ref-1 Kit ELISA, apurinic/apyrimidinic endodeoxyribonuclease 1 Kit ELISA, APEX nuclease (multifunctional DNA repair enzyme) 1 Kit ELISA, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog Kit ELISA, DNA-(apurinic or apyrimidinic site) lyase Kit ELISA, apurinic/apyrimidinic endonuclease 1 Kit ELISA, APEX1 Kit ELISA, apex1 Kit ELISA, apex1.L Kit ELISA, CNG03640 Kit ELISA, CNH01280 Kit ELISA, nth Kit ELISA, Spirs_4119 Kit ELISA, Palpr_0103 Kit ELISA, Apex1 Kit ELISA

Sujet:

Gene Name: Apurinic/Apyrimidinic Endonuclease 1

Gene Aliases: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus